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NanoBRET™ Protein:Protein Interaction System

Instructions for Use of Product(s)
N1661, N1662, N1663, N2583, N2584, N2585, N1811, N1821, N1581, N1641

Literature # TM439

The NanoBRET™ Protein:Protein Interaction (PPI) System is a new configuration and approach to bioluminescence resonance energy transfer (BRET), a proximity-based assay that can detect protein interactions by measuring energy transfer from a bioluminescent protein donor to a fluorescent protein acceptor. The NanoBRET™ assay uses a NanoLuc® fusion protein as the energy donor and a fluorescently labeled HaloTag® fusion protein as the energy acceptor. The optimized blue-shifted NanoLuc® donor paired with the red-shifted HaloTag® acceptor minimizes spectral overlap within the assay for an improved signal:background ratio when calculating the NanoBRET™ ratio. Measure live-cell PPI using an endpoint or kinetic assay.

Summary of Changes
The following change was made to the 3/20 revision of this document:

  1. Updated the protocols, representative data and troubleshooting items to include instructions for using the NanoBRET™ Nano-Glo® Kinetic Detection System.
  2. Change the volume of transfection reagent used for the protocols in Section 5.A.

Printed in USA. Revised 3/20.