Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

NanoBRET™ BRD4/Histone H3.3 Interaction Assay

Instructions for Use of Product(s)
N1830

Literature # TM444

The NanoBRET™ BRD4/Histone H3.3 Interaction Assay monitors the interaction between human BRD4 (Bromodomain containing protein 4) and histone H3.3 in living cells. This technical manual describes optimized NanoBRET™ protocols specific for detecting the interaction of either full-length BRD4 protein (BRD4 FL) or the N-terminal BRD4 bromodomain (BRD4 BD1) with the histone H3.3 variant. In this assay, we have included both the full-length BRD4 and highly studied BD1 alone for users that may want to understand the contribution of the bromodomain alone in addition to the full-length protein.

Summary of Change
The following change was made to the 12/15 revision of this document:
Updated the Chroma emission filters to use with the PerkinElmer EnVision® Multilabel Reader.

Printed in USA. Revised 12/15.