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MagneGST™ Pull-Down System Technical Manual

Instructions for Use of Product(s)

Literature # TM249

The MagneGST™ Pull-Down System provides GSH-linked magnetic particles that allow simple immobilization of GST-fusion bait proteins from bacterial lysates, and an in vitro transcription/translation system for expression of prey proteins. Prey proteins are isolated using MagneGST™ (GSH)Particles containing the appropriate bait protein. The MagneGST™ Pull-Down System protocol can be used for detection of interactions between GST-fusion proteins expressed in bacterial lysates and prey proteins expressed in the TnT® System.

Summary of Change
The following change was made to the 5/17 revision of this document:
1. Removed expired patent statements.

Printed in USA. Revised 5/17