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MagneGST™ Pull-Down System

V8870o

GST Pull-Down Assay for Protein:Protein Interaction Analysis

  • Analyze protein interactions between GST-fusion proteins from bacterial lysates and prey proteins expressed in TnT® Systems

Size

Catalog number selected: V8870

$ 717.00 Your price: Log In

MagneGST™ Pull-Down System
80 reactions
$ 717.00
Your price: Log In

The MagneGST™ Pull-Down System is designed to detect protein interactions between GST-fusion proteins expressed in bacterial lysates and prey proteins expressed in the TnT® Systems. Prey protein synthesized in the TnT® Quick Coupled Transcription/Translation Reaction is captured using bait protein (GST-fusion protein) immobilized on MagneGST™ Particles. Nonspecifically bound proteins are washed away, and the prey protein is analyzed. Prey proteins can be detected by incorporating radioactively-labeled methionine in the TnT® Quick reaction, followed by SDS-PAGE and autoradiography or by incorporating the supplied non-radioactive methionine in the TnT® reaction and detecting by Western blotting with protein-specific antibodies (see figure).

Note: This product requires the use of a magnetic stand, which is not included.

For more information, see the Protocols & Applications Guide.

Application

  • GST pull-down assays for the analysis of protein:protein interactions.
6544MA.eps
Representation of pull-down assay using bacterial expression of bait protein and TnT® cell-free system for the expression of the prey protein.

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Methionine

L118A 1 x 50μl1mM

RQ1 RNase-Free DNase

M610A 1 x 1,000u View Product

Nuclease-Free Water

P119A 1 x 1,250μl View Product

MagneGST™ Cell Lysis Reagent

V481B 1 x 60ml

MagneGST™ Glutathione Particles

V861A 1 x 4ml View Product

MagneGST™ Binding/Wash Buffer

V870B 2 x 200ml

TnT® T7 Quick Master Mix

V8871 1 x 8 × 200μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

Patent Pending.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

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