The MagneGST™ Pull-Down System is designed to detect protein interactions between GST-fusion proteins expressed in bacterial lysates and prey proteins expressed in the TnT® Systems. Prey protein synthesized in the TnT® Quick Coupled Transcription/Translation Reaction is captured using bait protein (GST-fusion protein) immobilized on MagneGST™ Particles. Nonspecifically bound proteins are washed away, and the prey protein is analyzed. Prey proteins can be detected by incorporating radioactively-labeled methionine in the TnT® Quick reaction, followed by SDS-PAGE and autoradiography or by incorporating the supplied non-radioactive methionine in the TnT® reaction and detecting by Western blotting with protein-specific antibodies (see figure).
Note: This product requires the use of a magnetic stand, which is not included.
For more information, see the Protocols & Applications Guide.
- GST pull-down assays for the analysis of protein:protein interactions.