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Protein analysis

Protein Purification Kits

These convenient manual and automation-friendly protein purification kits and reagents include products for purification of His, GST, Biotin or HaloTag affinity tagged proteins, and magnetic-based systems for purification of antibodies based on protein A or protein G binding. We also offer accessories for protein purification, such as protease inhibitor cocktail and cell lysis reagent.

Affinity purification is a powerful method to enrich and purify a recombinant protein from a cell lysate. Affinity tags are placed either on the N- or C-terminus of the protein of interest. Recombinant tagged proteins are purified from cell lysates or supernatants by selective binding, followed by washing and elution of the purified protein. After purification the protein may be cleaved with a protease to remove the affinity tag.

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22 Products


Capture Method

Purification Accessories

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High Capacity Magne® Streptavidin Beads

Magnetic affinity beads for binding biotinylated antibodies and proteins from complex biological samples with low nonspecific binding.

V7820, V7830

Magne® HaloTag® Beads

Magnetic capture of HaloTag® fusion proteins for protein pull-downs and purification. Magnetics enable streamlined protein purification, eliminate multiple centrifugation steps.

G7281, G7282

Magne® Protein G and Magne® Protein A Beads

Magnetic affinity beads with Protein A or G for Ab purification from culture media, ascites and serum samples.

G7471, G7472, G7473, G8781, G8782, G8783

HaloTag® Protein Purification System

Enhanced expression and solubility of recombinant proteins with HaloTag® Ligands.

G6280, G6270

MagneGST™ Pull-Down System

Prey protein is captured using bait protein (GST-fusion protein) immobilized on MagneGST™ Particles. Nonspecifically bound proteins are washed away.

V8870

HaloTag® Mammalian Protein Purification System

Highly pure recombinant protein from mammalian cell cultures, including proteins expressed at low levels.

G6790, G6795, G6799

HaloTEV Protease

Fusion of HaloTag® protein and TEV protease enables immobilization on HaloLink™ Resin and removal in a single-step purification.

G6601, G6602

Streptavidin

Has very high affinity (Kd=10–15M) for the vitamin biotin. Each subunit binds one biotin molecule.

Z7041

MagZ™ Protein Purification System

Purify expressed, polyhistidine- or HQ-tagged proteins from TnT® Rabbit Reticulocyte Lysate reactions, free of hemoglobin.

V8830

HisLink™ Protein Purification Resin

Purify His- or HQ-tagged proteins from cleared lysate by standard column chromatography or from crude lysate in batch.

V8821, V8823

MagneGST™ Protein Purification System

Immobilized paramagnetic particles isolate GST-tagged protein directly from crude or cleared lysate by either a manual or automated procedure, with a magnetic stand.

V8600, V8603

FastBreak™ Cell Lysis Reagent

Efficient, gentle lysis of E. coli cultures without centrifugation or mechanical disruption.

V8571, V8572, V8573

MagneHis™ Ni Particles

Paramagnetic precharged nickel particles used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate.

V8560, V8565

MagneHis™ Protein Purification System

Purification of His- or HQ-tagged, proteins from a crude cell lysate on paramagnetic particles with either a manual (requires use of a magnetic stand) or automated procedure.

V8500, V8550

Streptavidin Alkaline Phosphatase

For detecting biotinylated molecules.

V5591

Showing 22 of 22 Products

Protein Purification Kit Basics

There are numerous affinity tags that can be used for protein purification. Affinity tags vary greatly based on size, strength-of-binding and effect on protein solubility and stability. Promega protein purification kits are available for affinity purification using His, GST, HaloTag and Streptavidin/Biotin binding.

The most commonly used tag to purify recombinant proteins is the polyhistidine tag. Protein purification using His tags relies on the affinity of histidine residues for immobilized chelated metal such as nickel, which allows selective protein purification.  His tags offer several advantages for protein purification. The small size of the His tag makes it less immunogenic than larger tags. We offer scalable HisLink™ Systems for purification of His or HQ-tagged proteins from cell lysates.

The use of the affinity tag glutathione-S-transferase (GST) is based on the strong affinity of GST for immobilized glutathione-covered matrices. Glutathione-S-transferases are a family of multifunctional cytosolic proteins that are present endogenously in eukaryotic organisms but normally not found in bacteria. As such, GST-based purification is not recommended for eukaryotic systems (e.g., insect, mammalian). After capture and washing, the tagged protein is eluted with soluble glutathione. The MagneGST™ Protein Purification and Pull-Down Systems are designed for purification of GST-tagged proteins.

For protein purification HaloTag® Technology is compatible with many protein expression systems and can be applied to proteins expressed in E. coli, mammalian cells and cell-free systems. HaloTag® protein is a good affinity tag for purification and also a good solubility tag. The lack of an endogenous equivalent of the HaloTag® protein in mammalian and most prokaryotic cells minimizes the chances of nonspecific interactions. The combination of covalent capture with rapid binding kinetics overcomes the equilibrium-based limitations associated with traditional affinity tags, and enables efficient capture even at low expression levels.

The power of the streptavidin-biotin interaction is used in a number of applications, including the detection of various biomolecules. Streptavidin has a very high binding affinity for biotin. We offer Magne® Streptavidin Beads for purification of biotinylated antibodies, as well as Streptavidin Alkaline *hosphatase for detection of biotinlated proteins, and the PinPoint™cloning system for generation of biotinlyated fusion proteins. 

We offer a complete portfolio of protein analysis products supported by a growing library of educational resources, including technology guides, on-demand webinars, scientific articles and protocols.

Use the links below to learn more about protein analysis products, or to explore learning resources covering topics such as in vitro protein expression, protein purification methods and live-cell protein interaction analysis.