Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Fc Effector Activity Bioassays

Our ADCC and ADCP Reporter Bioassays are biologically relevant, MOA-based assays that can be used to measure the potency and stability of antibodies and other biologics that specifically bind and activate Fcγ receptors. The assays consist of Jurkat cells stably expressing the relevant Fcγ receptor variant and a luciferase gene regulated by the NFAT response element. 

The bioassays overcome the limitations of more labor-intensive and variable primary cell assays. The workflow is simple, scalable, and provides a quantitative measure of ADCC and ADCP with low variability and high accuracy. 

Save 20% on Detection Instruments to Optimize Your Assays ›

Filter By


Fc Effector Bioassays

Shop all Fc Effector Activity Bioassays

Showing 6 of 6 Products

Introduction to Fc Effector Activity

Fc receptors are proteins expressed on the surface of immune cells that contribute to the protective functions of the immune system. By binding antibodies attached to the surface of target cells, Fc receptors initiate cytotoxic or phagocytic effector cell activity. 

Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action of antibodies that target virus-infected or diseased (e.g., tumor) cells for destruction by components of the cell-mediated immune system. Fc receptor-mediated effects contribute to the efficacy and safety of a variety of therapeutic antibodies. Human FcγRIII is the predominant receptor involved in ADCC and exists as a high-affinity (FcγRIIIa-V158) or low-affinity (FcγRIIIa-F158) variant, depending on the amino acid at position 158. 

Antibody-dependent cell mediated phagocytosis (ADCP) is another critical mechanism of action for internalization and degradation of target cells by effector cells. Although other Fcγ receptors contribute, FcγRIIa is believed to be the predominant Fcγ receptor involved in ADCP.

Assays that can simply and reproducibly assess ADCC or ADCP are crucial in developing biologic therapeutics that function via these distinct mechanisms of action.