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Flexi® Rabbit Reticulocyte Lysate System

L4540o

Optimized Translation for Greater Protein Production

  • Increase protein production as much as fourfold over standard lysate reactions
  • For easy optimization the endogenous magnesium concentration is provided for each lot of Flexi® Lysate
  • The Flexi® System contains three Amino Acid Mixtures, providing a choice of radioisotopes

Size

Catalog number selected: L4540

$ 216.00 Your price: Log In

Flexi® Rabbit Reticulocyte Lysate System
30 reactions
$ 216.00
Your price: Log In

Translation Optimization for Mg2+ and K+ Concentrations

The Flexi® Rabbit Reticulocyte Lysate System allows translation reactions to be optimized for a wide range of parameters, including Mg2+ and K+ concentrations and the choice to add or omit DTT. To help optimize Mg2+ for a specific message, the endogenous Mg2+ concentration of each lysate batch is stated in the product information included with this product. The Flexi® System also offers the choice of three amino acid mixtures and includes a control RNA encoding the firefly luciferase gene.

The Flexi® Rabbit Reticulocyte Lysate  System, when optimized, can increase the quantity of protein produced as much as fourfold over a standard lysate reaction. The Flexi® System contains three Amino Acid Mixtures, enabling different choices of radioisotopes. Luciferase Control RNA and Luciferase Assay Reagent are included with the system as a functional control (only full-length luciferase is active).

For more information, see the Protocols & Applications Guide.

Applications:

  • Optimizing translation of low-expressing messages.
  • Oxidizing environment can be adjusted, which may be important for maintaining functional activity related to disulfide bridges.
  • Luciferase activity can be used for the detection of inhibitory contaminants in RNA preparations.
  • Investigation of translational control.
  • Screening of chemical libraries for effect on translation.

Reference:

  1. Beckler, G.S. et al. (1995) Methods in Molecular Biology 37, 215–32.

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Amino Acid Mixture Minus Cysteine

L447C 1 x 50μl1mM View Product

Flexi® Rabbit Reticulocyte Lysate

L455A 5 x 200μl

Luciferase Control RNA

L456A 1 x 10μl View Product

Luciferase Assay Wells

L457A 1 x 3 each

Magnesium Acetate

L458A 1 x 100μl25mM

Potassium Chloride

L459A 1 x 200μl2.5M

Luciferase Assay Reagent

L486A 1 x 250μl

Amino Acid Mixture Minus Leucine

L995A 1 x 50μl1mM View Product

Amino Acid Mixture Minus Methionine

L996A 1 x 50μl1mM View Product

DTT

P117A 1 x 100μl100mM View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Technical Bulletin for detailed storage information.

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