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Transcend™ Non-Radioactive Translation Detection System

Non-Radioactive Detection of In Vitro Translated Proteins

  • Biotin tag allows detection of 0.5–5ng of protein 3–4 hours after gel electrophoresis
  • No radioisotope handling, storage or disposal issues

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Catalog number selected: L5070

$ 312.00
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Transcend™ Non-Radioactive Translation Detection System
30 reactions
$ 312.00
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Non-radioactive detection of proteins synthesized in vitro. Using this system, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [35S]methionine or other radioactive amino acids. Biotinylated lysine is added to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA complex (Transcend™ tRNA) rather than as a free amino acid. After SDS-PAGE and electroblotting, biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish Peroxidase (Streptavidin-HRP), followed by colorimetric detection. Typically, this system enables you to detect 0.5–5ng of protein in 3–4 hours after gel electrophoresis. This sensitivity is equivalent to that achieved with [35S]methionine incorporation and autoradiographic detection 6–12 hours after gel electrophoresis.

For more information, see the Protocols & Applications Guide.

Structure of Transcend™ tRNA.

Structure of Transcend™ tRNA.
Structure of Transcend™ tRNA.

Specifications

What's in the box?

Item Part # Size Available Separately

Transcend™ tRNA

L506A 1 × 30μl View Product

Western Blue® Stabilized Substrate

S384C 1 × 35ml View Product

Streptavidin Alkaline Phosphatase

V559B 1 × 36μl View Product

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

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