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TnT® SP6 High-Yield Wheat Germ Protein Expression System

L3260_TnT SP6 High-Yield Wheat Germ Protein Expression System-40rxn_3
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Coupled Transcription/Translation for Soluble, Full-Length Protein in Only Two Hours

  • Express soluble, full-length protein from plasmid or PCR-generated templates quickly
  • Generate 10- to 20-fold more protein (10–100μg/ml) than with other cell-free systems

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Catalog number selected: L3260

$ 836.00
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TnT® SP6 High-Yield Wheat Germ Protein Expression System
40 reactions
$ 836.00
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Generate Soluble, Full-Length Protein in Two Hours

The TnT® SP6 High-Yield Wheat Germ Protein Expression System contains optimized wheat germ extract in a single-tube, coupled transcription/translation system that allows you to express proteins in only two hours from plasmid or PCR-generated templates. Protein is synthesized in concentrations in the range of 10–100μg/ml and can be used in multiple applications including high-throughput analysis.

All components necessary for transcription/translation are provided in the extract, with the exception of the plasmid DNA or PCR template. Optional protein-labeling reagents must also be supplied by the user.

For more information, see the Protocols & Applications Guide.

For custom wheat germ extract (depleted amino acids), order Cat.# X808X (see Products, Available Separately).

Applications

  • Protein:protein interactions
  • Protein:nucleic acid interactions
  • Protein:ligand interactions
  • Enzyme assays
  • Protein expression analysis

References

  1. Falah, N. et al. (2012) J. Virol. 86, 691–704.
  2. Stempin, C.C. et al. (2011) J. Biol. Chem. 286, 37147–57.
  3. Liu, W. et al. (2011) Plant Cell. 23, 3853–65.
  4. Shi, X. et al. (2011) J. Biol. Chem. 286, 33401–8.
  5. Zhao, L. et al. (2010) J. Struct. Funct. Genomics 11, 201–9.
  6. Zhao, K.Q., Hurst, R., Slater, M.R. and Bulleit, R.F. (2007) J. Struct. Funct. Genomics 8, 199–208.
9695ME-W

Cell-free protein expression from a variety of DNA sources in only two hours.

Specifications

You are viewing: L3260 Change Configuration

What's in the box?

Item Part # Size Available Separately

TnT® SP6 High-Yield Wheat Germ Master Mix

L326A 4 × 300μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L3261 Change Configuration

What's in the box?

Item Part # Size Available Separately

TnT® SP6 High-Yield Wheat Germ Master Mix

L326A 1 × 300μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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Uwe

Uwe

Germany