S30 T7 High-Yield Protein Expression System
Simplified Transcription/Translation of DNA Cloned in Plasmid or Lambda Vectors with a T7 Promoter
- Express up to 500μg/ml of protein in one hour
- Flexible detection of expressed proteins by Coomassie® staining or fluorescence or biotinylated labels
Catalog Number:
Size
Catalog Number: L1110
Catalog Number: L1115
High-Yield Protein Expression in One HourÂ
With the S30 T7 High-Yield Protein Expression System you can express up to 500μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site. The protein expression system provides an extract that contains T7 RNA polymerase for transcription and is deficient in OmpT endoproteinase and lon protease activity. All other necessary components in the system are optimized for protein expression. This results in greater stability and enhanced expression of target proteins.
Detect expressed proteins by Coomassie® staining or incorporation of a fluorescence or biotinylated modified tRNA. For more information, see the Protocols & Applications Guide.
Applications- Protein:protein interactions
- Protein:DNA interactions
- Enzyme activity
- Antibody production
Schematic of the protein expression system.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
S30 Premix Plus |
L111A | 3 × 200μl | |
|
S30 T7 Control DNA |
L112A | 1 × 20μl | |
|
T7/S30 Extract for Circular DNA |
L114A | 3 × 150μl | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
S30 Premix Plus |
L111A | 1 × 200μl | |
|
S30 T7 Control DNA |
L112A | 1 × 20μl | |
|
T7/S30 Extract for Circular DNA |
L114A | 1 × 150μl | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
