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TnT® Starter Bundle

Starter Pack for Protein Expression, Detection and Interactions Analysis

  • TnT® T7 Quick Coupled Transcription/Translation System, a simple one-hour, one-tube reaction
  • Transcend™ Translation Detection Systems detects proteins with colorimetric techniques
  • pTnT™ and pCMVTnT™ Vectors work to enhance cell-free expression
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Catalog number selected: L1215

$ 1,027.00
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TnT® Starter Bundle
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$ 1,027.00
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TnT® Starter Bundle

All the tools you need in one bundle to start cell-free expression and detection of your proteins. Use the system for in vitro analysis of protein:protein or protein:nucleic acid interactions, or simply verify the ability of your clone to express protein. Purchase this special bundle, and get the popular TnT® T7 Quick Coupled Transcription/Translation System and Transcend™ Translation Detection System, and receive two cell-free expression-qualified expression vectors, pTnT™ and pCMVTnT™ Vectors, at no extra cost. Each bundle includes:

TnT® T7 Quick Coupled Transcription/Translation System: Our most popular cell-free translation system—a simple one-hour, one-tube reaction. Requires only a protein coding sequence downstream of a T7 RNA polymerase promoter to produce protein. Produced protein may be used in a variety of applications including pull-downs, immunoprecipitations and gel shift assays. TnT® T7 Quick Coupled Transcription/Translation System Technical Manual #TM045.

Transcend™ Translation Detection Systems: A simple addition of the Transcend biotin-labeled lysine tRNA to the TnT® T7 Quick reaction provides a simple means of tagging a protein for easy detection. Detect proteins through simple Western blotting techniques with colorimetric techniques. Transcend™ Translation Detection Systems Technical Bulletin #TB182.

pTnT™ Vector: Specifically designed to work with the TnT® Systems with added features to enhance cell-free expression. pTnT™ Vector Technical Bulletin #TB304.

pCMVTnT™ Vector: Specifically designed to work with the TnT® Systems with added features to enhance cell-free expression. Go from cell-free expression to mammalian expression directly with built-in CMV promoter. pCMVTnT™ Vector Technical Bulletin #TB305.

Applications

  • GST pull-downs (view an animation).
  • Gel shift assays (view an animation).
  • Co-immunoprecipitations.
  • Characterization of protein modifications.
  • Protein:RNA interactions.
  • Protein activity studies.
  • Conformation of gene products.

Specifications

What's in the box?

Item Part # Size

TnT® T7 Quick Coupled Transcription/Translation System

L1170 1 × 40 reactions

Transcend™ tRNA

L5061 1 × 30μl

Transcend™ Colorimetric Translation Detection System

L5072 1 × 1 each

pTnT™ Vector

L5610 1 × 20μg

pCMVTnT™ Vector

L5620 1 × 20μg

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

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Manas

Manas

India