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Lumit® Immunoassay Cellular Systems

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No-Wash, One-Plate Immunoassay for Cellular Analytes

  • New Lumit® Lysis Buffer II included! Offers enhanced performance across a wide range of cellular targets
  • New Application Notes available for more validated targets
  • Set 1: Lumit® Anti-Mouse Ab-LgBiT/Lumit® Anti-Rabbit Ab-SmBiT
  • Set 2: Lumit® Anti-Mouse Ab-SmBiT/Lumit® Anti-Rabbit Ab-LgBiT
  • Starter Kit: Includes all four Set 1 and Set 2 Lumit® Secondary Antibodies; primary antibodies supplied by the user

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Catalog number selected: W1201

$ 394.00
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Lumit® Immunoassay Cellular Systems
Set 1/100 assays
$ 394.00
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Fast Quantification of Cellular Analytes

The Lumit® Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures target analytes directly in cell lysates. The assay can be adapted to detect a wide range of proteins, phosphoproteins or small molecules of interest, provided the appropriate primary antibodies (supplied by the user) are available. We recently developed a new lysis buffer (Lumit® Lysis Buffer II), which broadens the range of cellular targets (see table below) that can be interrogated using the Lumit® Immunoassay Cellular System.

Also available are Complete Assays, which include sets of antibodies for several important signaling nodes—pERK, pAKT, pSTAT3, pIκBα and pBTK as well as several others. These are available as early access materials.

To save you time, we have evaluated a number of common targets using commercially available primary antibodies. We have expanded the number of Application Notes to include targets that are now accessible due to the new lysis buffer. Our collection of application notes contains protocols, example data, optimized conditions and ordering information for each primary antibody.

Lists of Validated Targets

Cellular Pathway Analysis Series

*New Lysis Protocol using Lumit® Lysis Buffer II.

Human Cellular Target Signaling Pathway Lumit®
Immunoassay
Cellular
System
(Cat.#)
Application
Note
Phospho-4E-BP1 (Ser 65) mTor Set 2 (W1331) Download
AKT PI3K/mTOR/AKT Set 1 (W1201) Download
Phospho-AKT (Ser 473) PI3K/mTOR/AKT Set 1 (W1201) Download*
BCL6 B-cell signaling Set 1 (W1201) Download
β-catenin (Human) WNT Set 2 (W1331) Download
Phospho-β-catenin (Thr 41/Ser 45) WNT Set 2 (W1331) Download
Burton's Tyrosine Kinase (BTK) B-cell Receptor (BCR) Set 1 (W1201) Download
Phospho-BTK (Tyr 223) B-cell Receptor (BCR) Set 1 (W1201) Download
Phospho-CHK1 (Ser 317) DDR Pathway Set 1 (W1201) Download
Phospho-c-Jun (Ser 63) JNK Set 1 (W1201) Download
Phospho-c-MET (Tyr 1234/1235) c-MET Set 1 (W1201) Download
Phospho-c-MET (Tyr 1349) c-MET Set 1 (W1201) Download
CREB PKA/CREB Set 1 (W1201) Download
Phospho-CREB (Ser 133) PKA/CREB Set 1 (W1201) Download
Phospho-EGFR (Tyr 1068) EGFR Set 1 (W1201) Download
Phospho-EGFR (Tyr 1173) EGFR Set 1 (W1201) Download
ER (Estrogen Receptor) Estrogen Set 1 (W1201) Download
Phospho-ERK1 (Thr 202) ERK Set 1 (W1201) Download*
Phospho-GSK1-3β (Ser 9) GSK-3β Set 1 (W1201) Download
Phospho-H2AX DDR Pathway Set 1 (W1201) Download
Phospho-HER2 (Tyr 1196) HER2 Set 1 (W1201) Download
Phospho-HER2 (Tyr 1221/1222) HER2 Set 1 (W1201) Download
IκBα NF-κB Set 1 (W1201) Download
Phospho-IκBα (Ser 32) NF-κB Set 1 (W1201) Download
Phospho-JNK (Thr 183/Tyr 185) JNK Set 1 (W1201) Download*
p65 NF-κB Set 2 (W1331) Download*
Phospho-p65 (Ser 536) NF-κB Set 2 (W1331) Download*
Rb (Retinoblastoma Tumor Suppressor) Cell Cycle Set 2 (W1331) Download
Phospho-Rb (Retinoblastoma Tumor Suppressor) (Ser 780) Cell Cycle Set 2 (W1331) Download
Phospho-Rb (Retinoblastoma Tumor Suppressor) (Ser 807/811) Cell Cycle Set 2 (W1331) Download
Ribosomal Protein S6 PI3K/mTOR/AKT Set 1 (W1201) Download
Phospho-Ribosomal Protein S6 (Ser 240/244) PI3K/mTOR/AKT Set 1 (W1201) Download
Smad1 BMP Set 1 (W1201) Download
Phospho-Smad1 (Ser 463/465) BMP Set 1 (W1201) Download
Smad2 TGF-β Set 2 (W1331) Download
Phospho-Smad2 (Ser 465/467) TGF-β Set 2 (W1331) Download
STAT1 JAK/STAT Set 1 (W1201) Download
Phospho-STAT1 (Tyr 701) JAK/STAT Set 1 (W1201) Download
Phospho-STAT1 (Ser 727) JAK/STAT Set 1 (W1201) Download
Phospho-STAT2 (Tyr 690) JAK/STAT Set 1 (W1201) Download
STAT 3 JAK/STAT Set 1 (W1201) Download
Phospho-STAT 3 (Tyr 705) JAK/STAT Set 1 (W1201) Download

Targeted Protein Degradation Series

 

Human Cellular Target Degrader(s) Lumit®
Immunoassay
Cellular
System
(Cat.#)
Application
Note
AKT PROTAC MS-21 Set 1 (W1201) Download
B-cell lymphoma 6 protein (BCL-6) BI-3802 Set 1 (W1201) Download
BRD4 PROTACs ARV-771, dBET6 Set 1 (W1201) Download
Burton's Tyrosine Kinase (BTK) PROTAC MT-802 Set 1 (W1201) Download
ER (Estrogen Receptor) Fulvestrant Set 1 (W1201) Download
SMARCA2 (BRM) PROTAC ACBI-1 See Application Note for details Download
SMARCA4 (BRG1) PROTAC ACBI-1 Set 2 (W1331) Download
STAT3 PROTAC SD-36 Set 1 (W1201) Download

How the Lumit® Immunoassay Cellular System Works

Lumit® Immunoassay Cellular Systems include Lumit® Secondary Antibodies (rabbit and mouse; Lumit® Anti-Goat Antibody conjugates available separately), which are conjugated with NanoBiT® subunits: LgBiT and SmBiT. Binding of the antibody complexes brings LgBiT and SmBiT into close proximity to form an active NanoLuc® luciferase that produces light in proportion to the amount of target analyte.
See All Lumit® Secondary Antibodies
lumit immunoassay cellular system phosphoprotein detection

Simple One-Plate Protocol, No Wash Steps!

The Lumit® Immunoassay Cellular System does not require media removal or lysate transfer. The protocol simply involves addition of the labeled antibodies to the sample, addition of the detection reagent and reading of the luminescent signal—all in a single plate!

how-the-lumit-immunoassay-works-protocol

Lumit® Immunoassay vs. Western Blot

Lumit® Immunoassays are easier and much faster than traditional Western blots while generating comparable data.

Lumit immunoassay Wester blot protocol comparison
phospho-IkB lumit immunoassay vs Western blot
Total IkB lumit immunoassay vs Western blot

Quantitative Detection of Total and Phosphorylated Proteins

lumit-phospho-assay-ikb-p65

Using Lumit® Immunoassay Cellular Systems for Screening Novel Compounds in a Drug Discovery Process

Here, Dr. Tracey Martin from Cardiff University School of Medicine describes use of Lumit® Immunoassays in her work studying the motility protein N-WASP. The project involves screening 150 novel compounds to assess their effects on N-WASP expression and phosphorylation status. Multiple concentrations of each compound are tested in different cell types, generating a large number of samples. Originally the team were using Western blotting to look at N-WASP levels, however this was found to be too expensive and both labor and time-intensive.

Download Case Study

 

See More Data: This peer-reviewed paper published in Nature Communications Biology demonstrates how Lumit® Immunoassays were used to monitor multiple signaling pathway activation through specific nodes of phosphorylation (pIκBα, pAKT and pSTAT3).

Read the Paper
lumit-immunoassay-nature-communications-biology-paper

Specifications

You are viewing: W1201 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944C 1 × 50μl

Lumit® Detection Substrate C

VB103A 1 × 200μl

Lumit® Immunoassay Buffer C, 10X

VB115A 1 × 1.5ml

Lumit® Anti-Mouse Ab-LgBiT

W102A 1 × 30μl

Lumit® Anti-Rabbit Ab-SmBiT

W103A 1 × 30μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A 1 × 10μl

Lumit® Lysis Buffer II, 10X

VB310A 1 × 500μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1202 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 1 × 500μl

Lumit® Detection Substrate C

VB103B 2 × 1ml

Lumit® Immunoassay Buffer C, 10X

VB115B 1 × 15ml

Lumit® Anti-Mouse Ab-LgBiT

W102B 1 × 300μl

Lumit® Anti-Rabbit Ab-SmBiT

W103B 1 × 300μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A 2 × 10μl

Lumit® Lysis Buffer II, 10X

VB310B 1 × 5ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1203 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 10 × 500μl

Lumit® Detection Substrate C

VB103B 20 × 1ml

Lumit® Immunoassay Buffer C, 10X

VB115B 10 × 15ml

Lumit® Anti-Mouse Ab-LgBiT

W102B 10 × 300μl

Lumit® Anti-Rabbit Ab-SmBiT

W103B 10 × 300μl

Antibody Dilution Buffer

W108A 10 × 1ml

GF-AFC Substrate

G608A 20 × 10μl

Lumit® Lysis Buffer II, 10X

VB310B 10 × 5ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1331 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944C 1 × 50μl

Lumit® Detection Substrate C

VB103A 1 × 200μl

Lumit® Immunoassay Buffer C, 10X

VB115A 1 × 1.5ml

Lumit® Anti-Rabbit Ab-LgBiT

W104A 1 × 30μl

Lumit® Anti-Mouse Ab-SmBiT

W105A 1 × 30μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A 1 × 10μl

Lumit® Lysis Buffer II, 10X

VB310A 1 × 500μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1332 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 1 × 500μl

Lumit® Detection Substrate C

VB103B 2 × 1ml

Lumit® Immunoassay Buffer C, 10X

VB115B 1 × 15ml

Lumit® Anti-Rabbit Ab-LgBiT

W104B 1 × 300μl

Lumit® Anti-Mouse Ab-SmBiT

W105B 1 × 300μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A 2 × 10μl

Lumit® Lysis Buffer II, 10X

VB310B 1 × 5ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1333 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 10 × 500μl

Lumit® Detection Substrate C

VB103B 20 × 1ml

Lumit® Immunoassay Buffer C, 10X

VB115B 10 × 15ml

Lumit® Anti-Rabbit Ab-LgBiT

W104B 10 × 300μl

Lumit® Anti-Mouse Ab-SmBiT

W105B 10 × 300μl

Antibody Dilution Buffer

W108A 10 × 1ml

GF-AFC Substrate

G608A 20 × 10μl

Lumit® Lysis Buffer II, 10X

VB310B 10 × 5ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W1220 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944C 2 × 50μl

Lumit® Detection Substrate C

VB103A 2 × 200μl

Lumit® Immunoassay Buffer C, 10X

VB115A 2 × 1.5ml

Lumit® Anti-Mouse Ab-LgBiT

W102A 1 × 30μl

Lumit® Anti-Rabbit Ab-SmBiT

W103A 1 × 30μl

Lumit® Anti-Rabbit Ab-LgBiT

W104A 1 × 30μl

Lumit® Anti-Mouse Ab-SmBiT

W105A 1 × 30μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A 1 × 10μl

Lumit® Lysis Buffer II, 10X

VB310A 2 × 500μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

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