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Lumit™ Immunoassay Cellular Systems

No-Wash, One-Plate Immunoassay for Kinase Pathway Analysis

  • Set 1: Lumit™ Anti-Mouse Ab-LgBiT/Lumit™ Anti-Rabbit Ab-SmBiT
  • Set 2: Lumit™ Anti-Mouse Ab-SmBiT/Lumit™ Anti-Rabbit Ab-LgBiT
  • Starter Kit: Includes all four Set 1 & Set 2 Lumit™ Secondary Antibodies
  • Primary antibodies supplied by the user

Choose a System

Size

Catalog number selected: W1201

$ 350.00
Your price:
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This product is discontinued
Lumit™ Immunoassay Cellular Systems
Set 1/100 assays
$ 350.00
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Change Configuration

Fast Quantification of Phosphorylated or Total Protein

The Lumit™ Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures target analytes directly in cell lysates. The assay can be adapted to detect any phosphoprotein, total protein or small molecule of interest, provided that appropriate primary antibodies (supplied by the user) are available.

To save you time, we have evaluated a list of common targets using commercially available primary antibodies. Our collection of application notes contains protocols, example data, optimized conditions and ordering information for each primary antibody.

Find Your Target
Cellular Target Signaling Pathway Total Protein Protocol Phosphorylated Protein Protocol Lumit Immunoassay Cellular System Lumit Cat.# Application Note

AKT (Human)

PI3K/mTOR/AKT Yes Yes (Ser 473) Set 1 W1201 Download

4E-BP1 (Human)

mTor No Yes (Ser 65) Set 2 W1331 Download

β-catenin (Human)

WNT Yes Yes (Thr 41/ Ser 45) Set 2 W1331 Download

BTK (Human)

B-cell Receptor (BCR) No Yes (Tyr 223) Set 1 W1201 Download

c-Jun (Human)

JNK No Yes (Ser 63) Set 1 W1201 Download

CREB (Human)

PKA/CREB No Yes (Ser 133) Set 2 W1331 Download

Estrogen Receptor (ER- Human)

Estrogen Yes No Set 1 W1201 Download

IκBα (Human)

NF-κB
Yes Yes (Ser 32) Set 1 W1201 Download

JNK (Human)

JNK No Yes (Thr 183/Tyr 185) Set 1 W1201 Download

p65 (Human)

NF-κB
Yes Yes (Ser 536) Set 2 W1331 Download

Retinoblastoma Tumor Suppressor (Rb- Human)

Cell Cycle Yes Yes (Ser 780) Set 2 W1331 Download

Retinoblastoma Tumor Suppressor (Rb- Human)

Cell Cycle Yes Yes (Ser 807/811) Set 2 W1331 Download

Smad2 (Human)

TGF-β Yes Yes (Ser 465/Ser467) Set 2 W1331 Download

STAT 3 (Human)

JAK/STAT Yes Yes (Tyr 705) Set 1 W1201 Download

How the Lumit™ Immunoassay Cellular System Works

Lumit™ Immunoassay Cellular Systems include Lumit™ Secondary Antibodies (rabbit and mouse; Lumit™ Anti-Goat Antibody conjugates available separately), which are conjugated with NanoBiT® subunits: LgBiT and SmBiT. Binding of the antibody complexes brings LgBiT and SmBiT into close proximity to form an active NanoLuc® luciferase that produces light in proportion to the amount of target analyte.

See All Lumit™ Secondary Antibodies
lumit immunoassay cellular system phosphoprotein detection

Simple One-Plate Protocol, No Wash Steps!

The Lumit™ Immunoassay Cellular System does not require media removal or lysate transfer. The protocol simply involves addition of the labeled antibodies to the sample, addition of the detection reagent and reading of the luminescent signal—all in a single plate! 

how-the-lumit-immunoassay-works-protocol

Lumit™ Immunoassay vs. Western Blot

Lumit™ Immunoassays are easier and much faster than traditional Western blots while generating comparable data.
Lumit immunoassay Wester blot protocol comparison
phospho-IkB lumit immunoassay vs Western blot
Total IkB lumit immunoassay vs Western blot

Quantitative Detection of Total and Phosphorylated Proteins

lumit-phospho-assay-ikb-p65
Detection of total and phosphorylated IκBα and p65 upon NF-κB pathway activation and deactivation. Lumit™ Immunoassays revealed the predicted biology of NF-κB signaling pathway upon TNF treatment: IκBα phosphorylation (pS32) immediately followed by its fast degradation and p65 phosphorylation (pS536).

See More Data: This peer-reviewed paper published in Nature Communications Biology demonstrates how Lumit™ Immunoassays were  used to monitor multiple signaling pathway activation through specific nodes of phosphorylation (pIκBα, pAKT and pSTAT3).

Read the Paper
lumit-immunoassay-nature-communications-biology-paper

Specifications

You are viewing: W1201 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944C 1 × 50μl

Lumit™ Detection Substrate C

VB103A 1 × 200μl

Lumit™ Immunoassay Buffer C, 10X

VB115A 1 × 1.5ml

Lumit™ Anti-Mouse Ab-LgBiT

W102A 1 × 30μl

Lumit™ Anti-Rabbit Ab-SmBiT

W103A 1 × 30μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A-C 1 × 10μl

SDS

Choose language:

Download SDSPDF (397 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

Specifications

You are viewing: W1202 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 1 × 500μl

Lumit™ Detection Substrate C

VB103B 2 × 1ml

Lumit™ Immunoassay Buffer C, 10X

VB115B 1 × 15ml

Lumit™ Anti-Mouse Ab-LgBiT

W102B 1 × 300μl

Lumit™ Anti-Rabbit Ab-SmBiT

W103B 1 × 300μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A-C 2 × 10μl

SDS

Choose language:

Download SDSPDF (396 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

Specifications

You are viewing: W1203 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 10 × 500μl

Lumit™ Detection Substrate C

VB103B 20 × 1ml

Lumit™ Immunoassay Buffer C, 10X

VB115B 10 × 15ml

Lumit™ Anti-Mouse Ab-LgBiT

W102B 10 × 300μl

Lumit™ Anti-Rabbit Ab-SmBiT

W103B 10 × 300μl

Antibody Dilution Buffer

W108A 10 × 1ml

GF-AFC Substrate

G608A-C 20 × 10μl

SDS

Choose language:

Download SDSPDF (397 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

Specifications

You are viewing: W1331 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944C 1 × 50μl

Lumit™ Detection Substrate C

VB103A 1 × 200μl

Lumit™ Immunoassay Buffer C, 10X

VB115A 1 × 1.5ml

Lumit™ Anti-Rabbit Ab-LgBiT

W104A 1 × 30μl

Lumit™ Anti-Mouse Ab-SmBiT

W105A 1 × 30μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A-C 1 × 10μl

SDS

Choose language:

Download SDSPDF (398 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

Specifications

You are viewing: W1332 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 1 × 500μl

Lumit™ Detection Substrate C

VB103B 2 × 1ml

Lumit™ Immunoassay Buffer C, 10X

VB115B 1 × 15ml

Lumit™ Anti-Rabbit Ab-LgBiT

W104B 1 × 300μl

Lumit™ Anti-Mouse Ab-SmBiT

W105B 1 × 300μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A-C 2 × 10μl

SDS

Choose language:

Download SDSPDF (396 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

Specifications

You are viewing: W1333 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944D 10 × 500μl

Lumit™ Detection Substrate C

VB103B 20 × 1ml

Lumit™ Immunoassay Buffer C, 10X

VB115B 10 × 15ml

Lumit™ Anti-Rabbit Ab-LgBiT

W104B 10 × 300μl

Lumit™ Anti-Mouse Ab-SmBiT

W105B 10 × 300μl

Antibody Dilution Buffer

W108A 10 × 1ml

GF-AFC Substrate

G608A-C 20 × 10μl

SDS

Choose language:

Download SDSPDF (397 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

Specifications

You are viewing: W1220 Change Configuration

What's in the box?

Item Part # Size

Digitonin

G944C 2 × 50μl

Lumit™ Detection Substrate C

VB103A 2 × 200μl

Lumit™ Immunoassay Buffer C, 10X

VB115A 2 × 1.5ml

Lumit™ Anti-Mouse Ab-LgBiT

W102A 1 × 30μl

Lumit™ Anti-Rabbit Ab-SmBiT

W103A 1 × 30μl

Lumit™ Anti-Rabbit Ab-LgBiT

W104A 1 × 30μl

Lumit™ Anti-Mouse Ab-SmBiT

W105A 1 × 30μl

Antibody Dilution Buffer

W108A 1 × 1ml

GF-AFC Substrate

G608A-C 1 × 10μl

SDS

Choose language:

Download SDSPDF (446 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

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