The Lumit™ Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures target analytes directly in cell lysates. The assay can be adapted to detect any phosphoprotein, total protein or small molecule of interest, provided that appropriate primary antibodies (supplied by the user) are available.
Also available are Complete Assays, which include sets of phospho-specific antibodies for several important signaling nodes—pERK, pAKT, pSTAT3, pIκBα and pBTK. These are available as early access materials.
To save you time, we have evaluated a list of common targets using commercially available primary antibodies. Our collection of application notes contains protocols, example data, optimized conditions and ordering information for each primary antibody.