Insulin and glucagon are key target analytes measured in metabolism research. Secreted from β and α cells of pancreatic islets, respectively, the two hormones are vital for regulating glucose levels in the body. Using the Lumit™ Insulin and Lumit™ Glucagon Immunoassays, it is easy to collect hormone secretion data from monolayer cells in culture, 3D islet microtissues and islets. The simple add-and-read protocol is compatible with static and dynamic glucose-stimulated insulin secretion (GSIS) experiments, including perifusion systems.
Perifusion is a powerful method for studying hormone secretion over time and in response to sequential treatments, and it generates a large number of samples for analysis. Lumit™ Immunoassays make analysis of large sample sets like this much easier by avoiding tedious wash steps. The faster time to result gives you data sooner and enables you to move on to your next experiment.