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Lumit™ Glucagon Immunoassay

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No-Wash Immunoassay for Glucagon Detection

  • Fast add-and-read protocol
  • Luminescent detection with just a plate-reading luminometer
  • Flexible, scalable throughput: perform 100 assays in 96-well format or 400 assays in 384-well format using W8020; perform 500–2000 assays with W8022

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Catalog number selected: W8020

$ 815.00
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Lumit™ Glucagon Immunoassay
100–400 assays
$ 815.00
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Detect Glucagon Secretion Without Tedious Wash Steps

The Lumit™ Glucagon Immunoassay quantitatively measures glucagon from cell culture or islet secretion samples with a fast, easy, no-wash protocol. Simply add Lumit™ reagents to the sample, incubate and read using a standard multimode plate reader. Results are available in just 70 minutes. Lumit™ assays are ideal for quantitating hormone secretion from islet perifusion workflows. The flexible assay format can be scaled to 384-well plates for high-throughput sample processing.

How the Lumit™ Glucagon Immunoassay Works

Primary antibodies to glucagon, selected for their specificity and sensitivity, are labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of glucagon, the subunits are brought together to reconstitute active luciferase enzyme. Adding the optimized luciferase substrate generates a bright luminescent signal that is proportional to analyte levels.

How the Lumit™ Glucagon Immunoassay Works

Simple One-Plate Protocol, No Wash Steps

Lumit™ Glucagon Immunoassay Workflow

Plate Format

Glucagon Sample
Volume

Total Reaction
Volume

Number of Assays
(Cat.# W8020)

Number of Assays
(Cat.# W8022)

96-Well

50µl

125µl

100

500

384-Well

12.5µl

31.1µl

400

2000

Lumit™ assay setup is highly flexible and can be scaled up or down as desired. In addition to the configurations listed above, the assay is compatible with any sample volume ≥5µl provided proportionate amounts of Lumit™ reagents are used. For more information, see Table 1 in the Lumit™ Glucagon Immunoassay Technical Manual (#TM682).

Lumit™ Glucagon Immunoassay Has Broad Linear Range and Picomolar Sensitivity

The picomolar to nanomolar linear range accommodates popular cell models and islets.

Representative Titration Curve of Glucagon Positive Control

Specification

Lumit™ Glucagon Immunoassay

Limit of Detection (LOD)

~1pM (3.4pg/ml; 3 SD above background)

Linear Range

1pM–2nM

Signal/Background max

~500

Assay Time

70 minutes

Sample Type

Cell culture supernatants
Islet secretion samples

SD=Standard Deviation

Applications for the Lumit™ Immunoassays

Insulin and glucagon are key target analytes measured in metabolism research. Secreted from β and α cells of pancreatic islets, respectively, the two hormones are vital for regulating glucose levels in the body. Using the Lumit™ Insulin and Lumit™ Glucagon Immunoassays, it is easy to collect hormone secretion data from monolayer cells in culture, 3D islet microtissues and islets. The simple add-and-read protocol is compatible with static and dynamic glucose-stimulated insulin secretion (GSIS) experiments, including perifusion systems. 

Perifusion is a powerful method for studying hormone secretion over time and in response to sequential treatments, and it generates a large number of samples for analysis. Lumit™ Immunoassays make analysis of large sample sets like this much easier by avoiding tedious wash steps. The faster time to result gives you data sooner and enables you to move on to your next experiment.

Quantitate Insulin and Glucagon Secretion from Mouse Pancreatic Islets

Representative perifusion data showing insulin and glucagon secretion from mouse islets.

Insulin and glucagon secretion were measured in samples collected during perifusion experiments. Briefly, 80 mouse islets were placed in triplicate chambers of a perifusion instrument (Biorep, Miami Lakes, Florida). The islets were treated with 2.7mM glucose and then 10mM glucose, in combination with an amino acid mixture. Perifusate was collected every minute. Ten microliters of each sample were transferred into wells of a 384-well plate and assayed for either insulin or glucagon. Insulin and glucagon data sets are superimposed. This data was kindly provided by Drs. Hannah Foster and Matthew Merrins, University of Wisconsin VA Hospital, Madison, Wisconsin.

Featured Citation

In this work, El and colleagues use the Lumit™ Glucagon Immunoassay to characterize the complex regulatory mechanisms that control glucagon secretion from alpha cells.

El, K. et al. (2021) GIP mediates the incretin effect and glucose tolerance by dual actions on α cells and β cells. Sci. Adv. 7, abf1948.

Interview with the Author Read the Paper
lumit-glucagon-paper

Specifications

You are viewing: W8020 Change Configuration

What's in the box?

Item Part # Size

Lumit™ Detection Substrate B

VB405A 1 × 160μl

Lumit™ Detection Buffer B

VB406A 1 × 3.2ml

Lumit™ Antibody Dilution Buffer B

W150A 1 × 5.5ml

Glucagon Positive Control

W154A 1 × 25μl

Lumit™ Anti-Glucagon mAb-SmBiT, 400X

W155A 1 × 15μl

Lumit™ Anti-Glucagon mAb-LgBiT, 400X

W156A 1 × 15μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W8022 Change Configuration

What's in the box?

Item Part # Size

Lumit™ Detection Substrate B

VB405A 5 × 160μl

Lumit™ Detection Buffer B

VB406A 5 × 3.2ml

Lumit™ Antibody Dilution Buffer B

W150A 5 × 5.5ml

Glucagon Positive Control

W154A 5 × 25μl

Lumit™ Anti-Glucagon mAb-SmBiT, 400X

W155A 5 × 15μl

Lumit™ Anti-Glucagon mAb-LgBiT, 400X

W156A 5 × 15μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

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