We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Glucose Uptake-Glo™ Assay

A Simple, Non-Radioactive Assay to Measure Glucose Uptake Inside Cells

  • Homogeneous protocol
  • Achieve sensitivity with broad linearity
  • Reliable and reproducible results

Size

Catalog number selected: J1341

$ 350.00
Your price:
Add to Cart
This product is discontinued
Request a Sample
Not available in all countries
Add to Helix
Glucose Uptake-Glo™ Assay
5ml
$ 350.00
Your price: Log in
Change Configuration
GlucoseUptakeGloFullBackground-31311-PM

Glucose metabolism is a key process in many organisms. A lack of insulin-stimulated glucose uptake is associated with type 2 diabetes, while high glucose uptake is a sign of the high glycolytic rates associated with cancer. Measuring glucose uptake can determine the effects of various treatments for diabetes and cancer.

The gold standard method of assaying glucose uptake relies on detection of radio-labelled glucose analogs. Alternative colorimetric and fluorometric glucose uptake assays often lack the sensitivity and robustness needed to reliably measure glucose uptake in cells.

A Simple, Non-Radioactive Glucose Uptake Assay

The Glucose Uptake-Glo™ Assay is a plate-based, homogeneous bioluminescent method for measuring glucose uptake in cells, based on the detection of 2-deoxyglucose-6-phosphate (2DG6P).


A comparison of the Glucose Uptake-Glo Assay with the conventional radioactive method

2-deoxyglucose (2DG) is transported into cells and phosphorylated to produce 2-deoxyglucose-6-phosphate (2DG6P). The addition of Stop Buffer stops 2DG transport, lyses cells, destroys any NADPH within the cells and inactivates proteins. The addition of Neutralization Buffer neutralizes the solution before addition of the 2DG6P Detection Reagent. The glucose-6-phosphate dehydrogenase (G6PDH) within the reagent oxidizes 2DG6P to 6-phosphodeoxygluconate (6PDG) and reduces NADP+ to NADPH. The reductase uses the NADPH to convert the proluciferin to luciferin, which is then used by luciferase to produce light.

14130MA-W-a
14130MA-W-b

Measure Metabolic Changes in Cancer and Adipocyte Cell Lines

MCF7 Cancer Cells

In the cancer model, when cells are oxygen-starved, the hypoxic conditions shift cellular metabolism from oxidative phosphorylation to glycolysis. This results in increased glucose uptake.

MCF7 cells grown under hypoxia (1% oxygen) show an increase in Glucose Uptake-Glo™, indicating an increased glycolytic rate. The same cells demonstrate no significant change in viability using the RealTime-Glo™ and CellTiter-Fluor™ Assays.


Adipocyte Cells

Using the adipocyte model, we demonstrate that changes in glucose uptake for 3T3L1-MBX adipocytes did not have significant effects on cell health. Multiplexing the RealTime-Glo™ Assay with the Glucose Uptake-Glo™ Assay separates immediate effects on glucose uptake from global effects on cell health.

13506MB-W-a

Results of the Glucose Uptake-Glo™ Assay when adipocyte cells are treated under various conditions, where "None" indicates the basal level of glucose uptake. Insulin induces translocation of glucose transporters to the cell surface, and thus increases glucose uptake above basal levels. Cytochalasin B is a glucose transporter inhibitor which decreases glucose uptake. LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, is an essential insulin signaling enzyme which decreases glucose uptake relative to insulin alone.

13506MB-W-b

Results of the RealTime-Glo™ Assay when adipocyte cells are treated under various conditions, where "None" indicates the basal level of glucose uptake. Although there are dramatic changes in glucose uptake, as seen in the accompanying panel, there are minimal changes in viability as measured by the RealTime-Glo™ Assay.

Specifications

You are viewing: J1341 Change Configuration

What's in the box?

Item Part # Size

Luciferase Reagent

J151A 1 × 5ml

Stop Buffer

J152A 1 × 15ml

Neutralization Buffer

J153A 1 × 15ml

NADP+

J154A 1 × 50μl

G6PDH

J155A 1 × 125μl

2DG

J156A 1 × 250μl

2DG6P Standard

J157A 1 × 50μl

Reductase

G884C 1 × 25μl

Reductase Substrate

G885A 1 × 55μl

SDS

Choose language:

Download SDSPDF (244 KB) – English (United States)

Certificate of Analysis

See all certificates for J1341

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. No. 9,273,343 and other patents pending.

Specifications

You are viewing: J1342 Change Configuration

What's in the box?

Item Part # SizeConcentration

Luciferase Reagent

J151B 1 × 10ml

Stop Buffer

J152A 1 × 15ml

Neutralization Buffer

J153A 1 × 15ml

NADP+

J154B 1 × 100μl

G6PDH

J155B 1 × 250μl

2DG

J156A 1 × 250μl

2DG6P Standard

J157A 1 × 50μl

Reductase

G884A 1 × 55μl6mg/ml

Reductase Substrate

G885A 1 × 55μl

SDS

Choose language:

Download SDSPDF (244 KB) – English (United States)

Certificate of Analysis

See all certificates for J1342

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. No. 9,273,343 and other patents pending.

Specifications

You are viewing: J1343 Change Configuration

What's in the box?

Item Part # SizeConcentration

Luciferase Reagent

J151C 1 × 50ml

Stop Buffer

J152A 1 × 15ml

Neutralization Buffer

J153A 1 × 15ml

NADP+

J154C 1 × 500μl

G6PDH

J155C 1 × 1.25ml

2DG

J156A 1 × 250μl

2DG6P Standard

J157A 1 × 50μl

Reductase

G884B 1 × 275μl6mg/ml

Reductase Substrate

G885A 1 × 55μl

SDS

Choose language:

Download SDSPDF (244 KB) – English (United States)

Certificate of Analysis

See all certificates for J1343

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. No. 9,273,343 and other patents pending.

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-manos

Manos

UK