BCAA-Glo™ Assay

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Easy, Sensitive BCAA Assay Kit

  • Sensitive BCAA detection across broad linear range (50nM to 50µM)
  • Save time with in-well lysis; no cell collection or centrifugation required
  • Amenable to HTS applications

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Catalog number selected: CS306201

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BCAA-Glo™ Assay
5ml
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Efficient BCAA Detection from Diverse Samples

The BCAA-Glo™ Assay is a fast and sensitive method for detecting branched-chain amino acids (BCAA; leucine, isoleucine and valine) from various biological samples, such as cultured cells, media, tissue homogenates and serum. Based on bioluminescent technology, the BCAA-Glo™ Assay can detect subtle changes in amino acid metabolism and abundance. Its streamlined sample preparation protocol involves acid treatment and neutralization directly in the assay wells, eliminating the need for cell collection, centrifugation and spin columns. The assay is adaptable to a 384-well format, facilitating quick analysis of many samples.

How the BCAA-Glo™ Assay Works

Diagram illustrating how the BCAA-Glo™ Assay works.

Leucine Dehydrogenase catalyzes the oxidation of BCAA, simultaneously reducing NAD+ to NADH. In the presence of NADH, Reductase converts the proluciferin Reductase Substrate into luciferin. This reaction is detected by Ultra-Glo™ Recombinant Luciferase, which emits light proportionate to the amount of BCAA in the sample.

Leucine Dehydrogenase recognizes all three BCAA with similar efficiency. It is specific for BCAA L-isomers.

Simple Add-and-Read Protocol Saves Time

Diagram illustrating the BCAA-Glo™ Assay protocol workflow.

Accurately Detect the Smallest Changes in Branched-Chain Amino Acids

Graph showing the linear relationship between luminescence and leucine concentration.

 

Sensitivity (S/B >5)

400nM

Limit of Detection (S/N >3)

50nM

Linear Range

50nM to 25μM

Maximum Assay Window (S/B)

>300

Sensitive Quantitation of BCAA in Cultured Cells

Plot showing the linear relationship between luminescence and HCT116 and SKOV-3 cell lysate samples as measured by the BCAA-Glo™ Assay.

Cellular BCAA levels vary depending on cell type, growth conditions and metabolic activity. Intracellular BCAA concentrations are typically low. However, the BCAA-Glo™ Assay, with its high sensitivity and wide linear detection range, successfully measures BCAA levels in cell lysate samples ranging from 5,000 to 40,000 cells per 50μl, as shown in the graph.

Do you want to learn more about our cellular energy metabolism tools and the innovative technology that powers these assays?

Explore the Cellular Energy Metabolism Portfolio

Specifications

SDS

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Certificate of Analysis

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Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Resources

Other Resources

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Frequently Used With

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

GloMax® Explorer System

Easy-to-use, flexible multimode detection system.

GM3500, GM3510

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