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Flexi® Cloning System

A0003

Directional Cloning of Protein-Coding Sequences

  • Choose from a variety of initial applications (e.g., bacterial protein, mammalian or cell-free protein expression) and then transfer the insert as required
  • Efficient insert transfer means direct use of recombinant clones with minimal time for screening background colonies
  • Adaptable to high-throughput formats for large screening projects

Choose a kit

Size

Catalog number selected: C8640

$ 285.00 Your price: Log in

Flexi® Cloning System
Entry/Transfer/5 entry and 20 transfer reactions
$ 285.00
Your price: Log in
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The Flexi® Vector System is a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions without the need to resequence. Once an ORF is cloned, conveniently transfer the inserts into a variety of vectors designed for multiple applications. No licensing fees or complicated transfer restrictions needed.

All Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for the successful ligation of the insert.

Unlike site-specific recombination vector systems, the Flexi® Vector Systems do not require appending multiple amino acids to the amino or carboxy termini of the protein of interest. In addition, the systems do not require an archival entry vector, and most applications allow direct entry into the vector suited to the experimental design.

C-terminal Flexi® Vectors allow expression of C-terminal-tagged proteins. While these vectors can act as acceptors of protein-coding regions flanked by SgfI and PmeI, they lack a PmeI site and contain a different blunt-ended site, EcoICRI. This joined sequence cannot be removed from the C-terminal Flexi® Vectors and transferred to other Flexi® Vectors.

4599MA.eps
Cloning a protein-coding region into the Flexi® Vectors.
4600MA.eps
Transfer of a protein-coding region between N-terminal or native Flexi® Vectors.

For more information about the HaloTag® Flexi® Vectors, please visit the HaloTag® Technology page.

Note: For access to 20,000 human and 15,000 mouse ORF clones with HaloTag® Technology in multiple mammalian expression vectors and custom cloning options, visit: www.genecopoeia.com and www.kasuza.or.jp.kop/dsearch-e

Protocols

Specifications

You are viewing: C8640 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Wizard® SV Minicolumns

A129C 1 × 10/pk

Collection Tubes

A130C 1 × 10/pk

Vacuum Adapter

A133C 1 × 5/pk View Product

Wizard® SV Gel and PCR Clean-Up System

A9280 1 × 10 preps

Flexi® System, Entry/Transfer

C8641 1 × 1 each

T4 DNA Ligase (HC)

M179A 1 × 500u

Nuclease-Free Water

P119A 1 × 1,250μl View Product

5X Flexi® Digest Buffer

R186A 1 × 1ml

2X Flexi® Ligase Buffer

R187A 1 × 500μl

Flexi® Enzyme Blend (SgfI & PmeI)

R188A 2 × 50μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for storage recommendations.

Specifications

You are viewing: C8820 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

T4 DNA Ligase (HC)

M179A 4 × 500u

Nuclease-Free Water

P119A 2 × 1,250μl View Product

5X Flexi® Digest Buffer

R186A 1 × 1ml

2X Flexi® Ligase Buffer

R187A 2 × 500μl

Flexi® Enzyme Blend (SgfI & PmeI)

R188A 4 × 50μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

Patent Pending.

For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

Licensed under U.S. Pat. No. 5,945,288.

Specifications

You are viewing: C9320 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

T4 DNA Ligase (HC)

M179A 2 × 500u

Nuclease-Free Water

P119A 1 × 1,250μl View Product

5X Flexi® Digest Buffer

R186A 1 × 1ml

2X Flexi® Ligase Buffer

R187A 1 × 500μl

Flexi® Enzyme Blend (SgfI & PmeI)

R188A 1 × 50μl View Product

Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

R190A 1 × 50μl View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

Patent Pending.

For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

Licensed under U.S. Pat. No. 5,945,288.

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