MagneHis™ Protein Purification System provides a simple, rapid, reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Using a tube format, polyhistidine- or HQ-tagged protein can be purified on a small scale using less than 1ml of culture or on a large scale using more than 1 liter of culture. Samples can be processed in a high-throughput manner using a robotic platform such as the Beckman Coulter Biomek® 2000 or FX or Tecan Genesis® RSP.
MagneHis™ Ni-Particles are compatible with a variety of common buffers and are suitable for use with cultures volumes from 1ml–1 liter. Binding capacity is approximately 1mg of polyhistidine-tagged protein per 1ml of MagneHis™ Ni-Particles.
Note: This product requires the use of a magnetic stand.
For more information, see the Protocols & Applications Guide.
- Protein purification of polyhistidine- or HQ-tagged, expressed proteins from a crude E. coli cell lysate.
- High-throughput purification of His- or HQ-tagged proteins using automated platforms.
- Purification of His-tagged proteins from insect or mammalian cell lysates.
- Pull-down of His-tagged proteins for protein:protein interaction studies.