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MagneHis™ Protein Purification System

Magnetic Purification of Polyhistidine- or HQ-tagged Proteins from E. coli or Mammalian Cell Lysates

  • No centrifugation or vacuum required after cell lysis
  • Particles are compatible with a variety of common buffers
  • Binding capacity up to 1mg of polyhistidine-tagged protein per 1ml of particles

Size

Catalog number selected: V8500

$ 305.00 Your price: Log In

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MagneHis™ Protein Purification System
65 reactions
$ 305.00
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Purify Polyhistidine (His-) or HQ-Tagged Proteins

MagneHis™ Protein Purification System provides a simple, rapid, reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Using a tube format, polyhistidine- or HQ-tagged protein can be purified on a small scale using less than 1ml of culture or on a large scale using more than 1 liter of culture. Samples can be processed in a high-throughput manner using a robotic platform such as the Beckman Coulter Biomek® 2000 or FX or Tecan Genesis® RSP.

MagneHis™ Ni-Particles are compatible with a variety of common buffers and are suitable for use with cultures volumes from 1ml–1liter. Binding capacity is approximately 1mg of polyhistidine-tagged protein per 1ml of MagneHis™ Ni-Particles.

Note: This product requires the use of a magnetic stand.

For more information, see the Protocols & Applications Guide.

Applications
  • Protein purification of polyhistidine- or HQ-tagged, expressed proteins from a crude E. coli cell lysate.
  • High-throughput purification of His- or HQ-tagged proteins using automated platforms.
  • Purification of His-tagged proteins from insect or mammalian cell lysates.
  • Pull-down of His-tagged proteins for protein:protein interaction studies.

 

 

3922TB.eps
Purification of His-tagged methionyl tRNA synthetase.

Protocols

Specifications

You are viewing: 65 reactions Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

MagneHis™ Binding/Wash Buffer

V851A 1 x 50ml

MagneHis™ Elution Buffer

V852A 1 x 10ml

MagneHis™ Ni-Particles

V854A 1 x 2ml View Product

FastBreak™ Cell Lysis Reagent, 10X

V857A 1 x 15ml View Product

DNase I (lyophilized)

Z358B 1 x 1 vial

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

U.S. Pat. Nos. 7,112,552 and 7,354,750.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Specifications

You are viewing: 325 reactions Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

MagneHis™ Binding/Wash Buffer

V851B 1 x 250ml

MagneHis™ Elution Buffer

V852B 1 x 50ml

MagneHis™ Ni-Particles

V854B 1 x 10ml View Product

FastBreak™ Cell Lysis Reagent, 10X

V857B 1 x 60ml View Product

DNase I (lyophilized)

Z358A 2 x 1 vial

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

U.S. Pat. Nos. 7,112,552 and 7,354,750.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

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