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CellTox™ Green Cytotoxicity Assay

32459740-CellToxGreen_PosterImage_1280x720

Real-Time Cytotoxicity Detection Assay With Multiplexing Compatibility

  • Accurate cytotoxicity determination in exposures out to 72 hours
  • Flexible protocol allows kinetic analysis or endpoint determination
  • Multiplex with luminescent assays to obtain more data per well

Size

Catalog number selected: G8741

$ 115.00 Your price: Log in

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CellTox™ Green Cytotoxicity Assay
10ml
$ 115.00
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Interpreting Multiplexing Data Using the CellTox™ Green Cytotoxicity Assay

Cytotoxicity assays based on detection of biomarkers released into the media can underestimate cytotoxicity in long-term exposures of 72 hours or more because of limited stability of the biomarker detected. The CellTox™ Green Cytotoxicity Assay provides an easy, fast and accurate method to determine toxic effects during or after long-term exposure of cells in culture. CellTox Green can be combined with other methods in multiplex assays to determine mechanism of toxicity, and is easily scalable from 96- to 1536-well plate formats.

10887MB-W

CellTox™ Green Dye binds DNA of cells with impaired membrane integrity.

Perform Kinetic Cytotoxicity Measures to Determine Onset of Toxicity

Dose- and exposure-dependent increases in cytotoxicity

In the experiment shown here, bortezomib caused appreciable cytotoxicity in K562 cells between the 4- and 24-hour time points. Additive fluorescence at later time points indicates continued loss of membrane integrity occurring as a function of cytotoxicity against remaining cells. The CellTox™ Green Dye was added at seeding. 

11114ME-W

Multiplex for More Informative Data per Well

Measure Cytotoxicity and Viability

Here, CellTox™ Green Dye was mixed with K562 Cells, which were plated, then dosed with compound. CellTiter-Glo® Cell Viability Assay Reagent was added at the end of the 72-hour exposure and luminescence (viability) measured. These two inverse measures of cell health resulted in EC50 agreement.

10935MB-W

Determine Appropriate Timing of Caspase Assays

CellTox™ Green can be used in kinetic mode to determine the onset of cytotoxicity followed by multiplexing with the Caspase-Glo® 3/7 Assay in the same sample well to confirm caspase-3/7 activation at the appropriate time.

11115MB-W

Differentiate Between Cytotoxic and Cytostatic Effects

In this example, K562 cells were exposed to Methotrexate, an anti-proliferative compound, for 72 hours. CellTox™ Green Dye was added to determine cytotoxicity, then CellTiter-Glo® Reagent was added to the same sample well for viability determination. A viability assay alone would have misreported the compound as cytotoxic due to less cells in the treated sample compared to the non-treated control, which continued to proliferate. Multiplexing with a cytotoxicity assay reveals no change in signal as a result of compromised membrane integrity, indicating growth inhibition and not cytotoxicity.

11589TC-W

Specifications

You are viewing: G8741 Change Configuration

What's in the box?

Item Part # Size

Lysis Solution

G182B 1 × 0.5ml

Assay Buffer

G610A 1 × 10ml

CellTox™ Green Dye, 1,000X

G873A 1 × 20μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G8742 Change Configuration

What's in the box?

Item Part # Size

Lysis Solution

G182B 1 × 0.5ml

Assay Buffer

G610B 1 × 50ml

CellTox™ Green Dye, 1,000X

G873A 5 × 20μl

SDS

Choose language:

Certificate of Analysis

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View more results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G8743 Change Configuration

What's in the box?

Item Part # Size

Lysis Solution

G182B 1 × 0.5ml

Assay Buffer

G610B 2 × 50ml

CellTox™ Green Dye, 1,000X

G873B 1 × 200μl

SDS

Choose language:

Certificate of Analysis

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View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G8731 Change Configuration

What's in the box?

Item Part # Size

CellTox™ Green Dye, 1,000X

G873B 1 × 200μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

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