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CytoTox-ONE™ Homogeneous Membrane Integrity Assay

A Fluorometric Method for Estimating the Number of Nonviable Cells Present in Multiwell Plates

  • Elimination of cell transfer step saves time
  • Perform multiple assays on one sample

Size

Catalog number selected: G7890

$ 155.00 Your price: Log In

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CytoTox-ONE™ Homogeneous Membrane Integrity Assay
200–800 assays
$ 155.00
Your price: Log In
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The CytoTox-ONE™ Homogeneous Membrane Integrity Assay is a fluorometric method for estimating the number of nonviable cells present in multiwell plates. The assay rapidly measures the release of lactate dehydrogenase (LDH) from cells with a damaged membrane. LDH released into the culture medium is measured with a 10-minute coupled enzymatic assay that results in the conversion of resazurin into a fluorescent resorufin product. The amount of fluorescence produced is proportional to the number of lysed cells using a 96- or 384-well format. The CytoTox-ONE™ Reagent does not damage normal healthy cells; therefore the reactions set up to measure released LDH can be performed directly in a homogeneous format in assay wells containing a mixed population of viable and damaged cells.

The CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP (Cat.# G7892), offers convenient, alternative packaging for processing multiple plates. Each bottle of reagent supplied with the system is sufficient to perform 500 assays in a 96-well format or 2,000 assays in a 384-well format when the recommended volumes are used.

Save time by setting up the assay in a cell culture plate; this eliminates the sample transfer step common in many LDH assays. Plates are then incubated for just 10 minutes before reading data, compared to 30 minutes or more with classic LDH assays.

Multiplexing capability allows you to perform multiple assays on one sample with other homogeneous cell-based assays from Promega.

Adapt the assay protocol to your needs; completed assays can be read over several hours after the provided stop solution has been added while still maintaining good signal.

Protocols

Specifications

You are viewing: 200–800 assays Change Configuration

What's in the box?

Item Part # Size

Substrate Mix

G179A 2 x 1.5ml

Lysis Solution

G182B 1 x 0.5ml

Assay Buffer

G790A 1 x 24ml

Stop Solution

G791A 1 x 11ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

U.S. Pat. Nos. 6,982,152 and 7,282,348 and other patents.

Patent Pending.

Specifications

You are viewing: 1,000–4,000 assays Change Configuration

What's in the box?

Item Part # Size

Substrate Mix

G179A 10 x 1.5ml

Lysis Solution

G182B 1 x 0.5ml

Assay Buffer

G790B 1 x 120ml

Stop Solution

G791B 1 x 60ml

SDS

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Certificate of Analysis

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View more results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,982,152 and 7,282,348 and other patents.

Patent Pending.

Specifications

You are viewing: 1,000–4,000 assays Change Configuration

What's in the box?

Item Part # Size

Substrate Mix

G179B 2 x 7.5ml

Lysis Solution

G182B 1 x 0.5ml

Assay Buffer

G790B 1 x 120ml

Stop Solution

G791B 1 x 60ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,982,152 and 7,282,348 and other patents.

Patent Pending.

Resources

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