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DNA Purification & Transfection

Name Description Part Number
PURIFICATION
PureYield Plasmid Maxiprep System Open/Close Add
One-hour, vacuum-based protocol
Highly pure, concentrated plasmid DNA
No tedious, gravity-drip columns or post-elution alcohol precipitation

The PureYield Plasmid Maxiprep System isolates transfection-quality plasmid DNA, providing a rapid method to purify up to 1mg of plasmid DNA from a 250ml bacterial culture. Purify plasmid DNA rapidly with the vacuum protocol, greatly reducing time spent compared to silica resin or other membrane-column methods. This system incorporates a unique Endotoxin Removal Wash to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Purify without isopropanol precipitation. Use the Eluator Vacuum Elution Device to elute nucleic acids from PureYield Midiprep or Maxiprep columns. The device base interfaces with a Vacuum Manifold that contains Luer-Lok fittings, such as the Vac-Man Laboratory Vacuum Manifold (Cat.# A7231), and holds a 1.5ml tube to capture the eluted nucleic acids. The column assembly accepts PureYield Midiprep or Maxiprep columns. The device eliminates the need for a centrifuge with a swinging bucket rotor for nucleic acid purification.

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PureYield Plasmid Midiprep System Open/Close Add
30-minute, vacuum-based protocol
Highly pure, concentrated plasmid DNA
No tedious, gravity-drip columns or post-elution alcohol precipitation

The PureYield Plasmid Midiprep System isolates transfection-quality plasmid DNA, providing a rapid method to purify 100-200ug of plasmid DNA from a 50ml bacteria culture. Purify plasmid DNA in as little as 30 minutes with the vacuum protocol, greatly reducing time spent compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400ug of high-copy-number plasmid from 250ml of E. coli culture. The system incorporates a unique Endotoxin Removal Wash to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Purify without isopropanol precipitation or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man Laboratory Vacuum Manifold).

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PureYield Plasmid Miniprep System Open/Close Add
Rapid, 10-minute miniprep protocol
Highly pure, concentrated plasmid DNA
No tedious, gravity-drip columns or post-elution alcohol precipitation

The PureYield Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. The system provides a rapid method for purification of up to 15ug of plasmid DNA from 600ul to 3ml of bacteria culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TNT Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man Laboratory Vacuum Manifold).

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Wizard MagneSil Plasmid Purification System Open/Close Add
Process multiple plates without user intervention
Validated automated methods available

The Wizard MagneSil Plasmid DNA Purification System provides a simple and reliable method for the rapid isolation of plasmid DNA in a 96-well, high-throughput format. The purified plasmid can be used directly for automated fluorescent sequencing, such as with BigDye terminator sequencing chemistry, as well as for other standard molecular biology techniques including restriction enzyme digestion. The use of the MagneSil Paramagnetic Particles for lysate clearing (BLUE) as well as DNA capture (RED) circumvents the need for centrifugation or vacuum manifolds, making the system ideal for full automation on a Beckman Coulter or Tecan instrument.

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Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems Open/Close Add
60-minute protocol
Manual and automated formats
Vacuum-based method

The Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems provide a simple and reliable method for the rapid isolation of plasmid DNA using a silica-membrane, 96-well, high-throughput format. A single plate can be processed in 60 minutes or less. The purified plasmid can be used directly for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques, including restriction enzyme digestion. The Wizard SV 96 and SV 9600 Systems are designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments.

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Wizard SV Gel and PCR Clean-Up System Open/Close Add
Choice of spin- or vacuum-based protocols
Purifies 100bp to 10kb DNA fragments

The Wizard SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests. Up to 95% recovery is achieved depending upon the DNA fragment size. PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, which can bind up to 40ug of DNA, allows recovery of isolated DNA fragments or PCR products in as little as 15 minutes, depending on the number of samples processed. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation.

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x-tracta Gel Extractor Open/Close Add
Removes a 0.13 × 0.33 inch gel piece
Disposable device reduces cross-contamination
No need to use razor blades or scalpels

The x-tracta Gel Extractor tool provides a convenient, safe method for removal of agarose gel fragments for further processing. The device removes a 0.13 x 0.33 inch gel piece from agarose gels for easy transfer into a microcentrifuge tube for processing. The x-tracta tool eliminates the need for razor blades or scalpels, and its single-use design eliminates the possibility for sample-to-sample cross-contamination. Note: The x-tracta Gel Extractor works best on 0.6-2% analytical grade agarose gels. Please exercise caution if using the x-tracta Gel Extractor on Low Melting Point (LMP) agarose gels because the extractor does not work effectively due to the gel consistency.

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TRANSFECTION
FuGENE 6 Transfection Reagent Open/Close Add
Preserve cell viability
Excellent compatibility with everyday cell lines
Straightforward protocol; minimal optimization

FuGENE 6 Transfection Reagent is a nonliposomal formulation designed to transfect plasmid DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.

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FuGENE HD Transfection Reagent Open/Close Add
Difficult-to-transfect cell lines
Transfection of primary cells
Protein and virus production

FuGENE HD Transfection Reagent is a novel, nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE HD Transfection Reagent has been shown to support transfection in chemically defined media and does not contain any animal-derived components. The cell lines listed in Table 1 of TM328 have been successfully transfected by Promega Corporation or Fugent, L.L.C. For a list of conditions that were used in the transfection of these and other cell types, visit our FuGENE HD Protocol Database at: www.promega.com/techserv/tools/FugeneHD/

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ViaFect Transfection Reagent Open/Close Add
Performs better than or equal to other best-in-class transfection reagents
Simple transfection protocol with minimal optimization required
Low toxicity for routine and challenging cell lines

ViaFect Transfection Reagent is a novel formulation reagent designed to efficiently introduce DNA into a wide variety of cell lines. ViaFect Transfection Reagent has performed in commonly used adherent cell models and also in cells lines traditionally thought of as difficult to transfect such as suspension cells and stem cell-derived lines. This gentle, low-toxicity reagent allows cells to stay healthy and metabolically active during transfection-based experiments and offers an easy-to-use protocol that does not require removal of serum or culture medium prior to use. After introducing the reagent:DNA complex no washing or medium changes are required. ViaFect Transfection Reagent provides robust performance with minimal optimization allowing simple design of more relevant assays.

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