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CloneWeaver™ Workflow Builder

Powering Your Cloning Workflow

Product selection has never been easier

Molecular Cloning is the process of producing recombinant DNA and transforming into host organisms to replicate and make more copies. Every cloning project is unique. Once you have designed the cloning scheme, gathering all of the required reagents to get you from construct to expression and analysis is not trivial. CloneWeaver helps you build a customized cloning "kit" with all of the items your cloning scheme requires. Purchase your selection instantly, save it or email it to a purchasing agent. Soon you'll have everything you require to create the construct you need.

Select any of the product areas below to get started:

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Select Cloning Vector Type

Bacterial Vectors

Bacterial vectors contain the required genetic elements such as an Origin of Replication (ORI) to allow expression from bacterial hosts, such as E. coli. They can be high- or low-copy number depending on the goals of your experiment.

Cell Free Expression

Cell-free expression vectors are designed for use with Promega cell-free expression systems including rabbit reticulocyte lysate, wheat germ extract and insect cell extract-based systems.

Mammalian Expression

Mammalian expression vectors enable you to express cloned sequences in mammalian cells, and include multipurpose vectors, vectors for cloning and expression of PCR products, two-hybrid system vectors and vectors for regulated mammalian expression.

Mammalian Reporter

Use mammalian reporter vectors to create fusions to a reporter gene that acts as an indicator to study gene expression or cellular events in cells, tissues or whole cells.

PCR Cloning

These vectors allow you to conveniently clone PCR products directly without the need to modify insert with further enzymatic treatment before cloning. They are also called T-vectors because they contain T-overhangs to allow ligations of amplification products made by Taq DNA polymerases which leave A-overhangs, if they do not contain a proofreading activity.

RNAi

RNAi vectors are reporter vectors that enable study of miRNA or other small regulatory RNA activity by the insertion of a target sequence downstream of a reporter gene. RNAi vectors help provide a powerful genetic tool for selectively silencing genes. Generally RNAi vectors contain a minimal promoter so that you canvalidate the stoichiometric reaction of the two RNA molecules (target and miRNA) interacting with each other.

Subcloning

Subcloning vectors allow you to transfer a piece of inserted DNA from one vector to another to gain specific functionality. Subcloning vectors are selected based on your experimental goals: Do you need to express your DNA in mammalian cells or bacteria? Do you need to express large quantities of proteins or test a regulatory sequence?