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The S30 T7 High-Yield Protein Expression System

Kate Qin Zhao, Don Creswell and Michael Slater
Promega Corporation
Publication Date: 2008

Abstract

The S30 T7 High-Yield Protein Expression System is an E. coli extract-based cell-free protein synthesis system that transcribes and translates DNA sequences cloned in vectors containing a T7 promoter or a strong E. coli promoter. Protein yield can reach hundreds of micrograms per milliliter of reaction (up to 500µg/ml) within 1 hour at 37ºC. The reaction volume can be scaled down to as little as 5µl for high-throughput applications.

Promega Notes 100, 9–10.