S30 T7 High-Yield Protein Expression System Technical Manual
Literature # TM306
The S30 T7 High-Yield Protein Expression System is an E. coli extract-based cell-free protein synthesis system. It simplifies the transcription and translation of DNA sequences cloned in plasmid or lambda vectors containing a T7 promoter by providing an extract that contains T7 RNA polymerase for transcription and all necessary components for translation. This system can produce high levels of recombinant proteins (up to hundreds of micrograms of recombinant protein per milliliter of reaction) within an hour. The investigator supplies only cloned DNA containing a T7 promoter and a ribosome-binding site (RBS).
Summary of Change
The following change was made to the 5/16 revision of this document:
Corrected notes for protocol in Section 6.
Printed in USA. Revised 5/16.