PowerPlex® 18D System for Use on the Applied Biosystems® Genetic Analyzers Protocol
Literature # TMD031
The PowerPlex® 18D System allows co-amplification and four-color fluorescent detection of eighteen loci, including D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, Amelogenin, vWA, D8S1179, TPOX, FGA, D19S433 and D2S1338. The PowerPlex® 18D System provides all materials necessary to amplify STR regions of genomic DNA, including a hot-start, nonproofreading thermostable DNA polymerase. The system is optimized for analysis of common database samples, such as unwashed FTA® card punches (i.e., direct amplification). Additionally, thermal cycling time is reduced from previous PowerPlex® protocols. The PowerPlex® 18D System is compatible with the ABI PRISM® 3100 and 3100-Avant and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.
Summary of Changes
The following changes were made to the 4/17 revision of this document:
1. Added support for half-volume direct-amplification reactions and use of the Veriti® 96-Well Thermal Cycler.
2. Added caution against storing PCR amplification mix prior to thermal cycling.
3. Revised document name to specify that this manual is for use on Applied Biosystems instruments.
4. Incorporated other general updates.
Printed in USA. Revised 4/17.