Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

PowerPlex® 18D System for Use on the Applied Biosystems® Genetic Analyzers Protocol

Instructions for Use of Product(s)
DC1802, DC1808

Literature # TMD031

The PowerPlex® 18D System allows co-amplification and four-color fluorescent detection of eighteen loci, including D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, Amelogenin, vWA, D8S1179, TPOX, FGA, D19S433 and D2S1338. The PowerPlex® 18D System provides all materials necessary to amplify STR regions of genomic DNA, including a hot-start, nonproofreading thermostable DNA polymerase. The system is optimized for analysis of common database samples, such as unwashed FTA® card punches (i.e., direct amplification). Additionally, thermal cycling time is reduced from previous PowerPlex® protocols. The PowerPlex® 18D System is compatible with the ABI PRISM® 3100 and 3100-Avant and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

Summary of Changes
The following changes were made to the 4/17 revision of this document:
1. Added support for half-volume direct-amplification reactions and use of the Veriti® 96-Well Thermal Cycler.
2. Added caution against storing PCR amplification mix prior to thermal cycling.
3. Revised document name to specify that this manual is for use on Applied Biosystems instruments.
4. Incorporated other general updates.

Printed in USA. Revised 4/17.