The PowerPlex® 18D System is a multiplex STR system for use in database and paternity testing. This system is optimized for direct amplification of samples on FTA® cards in less than 1.5 hours using rapid cycling technology. This five-color multiplex allows co-amplification of the 13 CODIS loci (D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818) plus Amelogenin, Penta E, Penta D, D2S1338 and D19S433. All eighteen loci are amplified simultaneously in a single tube and analyzed in a single injection.
Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® ID and ID-X software and are available for download. The PowerPlex® 18D System is compatible with ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.