Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

NanoBRET™ Target Engagement Intracellular HDAC Assay Technical Manual

Instructions for Use of Product(s)
N2080, N2081, N2090, N2170

Literature # TM483

The NanoBRET™ Target Engagement (TE) Assay measures compound binding at select target proteins within intact cells by analyzing the apparent affinity of test compounds by competitive displacement of a NanoBRET™ tracer reversibly bound to a NanoLuc® fusion protein in cells. In the first step of the NanoBRET™ TE Assay, a fixed concentration of tracer is added to cells expressing the desired NanoLuc® fusion protein. Introduction of competing compounds results in a dose-dependent decrease in NanoBRET™ energy transfer for estimating intracellular affinity at the target protein. The NanoBRET™ TE Intracellular HDAC Assay assesses compound binding to a histone deacetylase (HDAC)-NanoLuc® luciferase fusion protein.

Printed in USA 6/16.