NanoBRET™ Target Engagement Intracellular BET BRD Assay Technical Manual
Literature # TM478
The NanoBRET™ Target Engagement (TE) Assay measures compound binding at select target proteins within intact cells by analyzing the apparent affinity of test compounds by competitive displacement of a NanoBRET™ tracer reversibly bound to a NanoLuc® fusion protein in cells. In the first step of the NanoBRET™ TE Assay, a fixed concentration of tracer is added to cells expressing the desired NanoLuc® fusion protein. Introduction of competing compounds results in a dose-dependent decrease in NanoBRET™ energy transfer for estimating intracellular affinity at the target protein. The NanoBRET™ TE Intracellular BET BRD Assay assesses compound binding to a bromodomain and extraterminal domain family (BET) bromodomain (BRD)-NanoLuc® luciferase fusion protein.
Printed in USA 6/16.