Griess Reagent System Technical Bulletin

Instructions for Use of Product(s)

Literature # TB229

The Griess Reagent System is based on the chemical diazotization reaction that was originally described by Griess in 1879, which uses sulfanilamide and N-1-napthylethylenediamine dihydrochloride (NED) under acidic (phosphoric acid) conditions. This system detects NO2 in a variety of biological and experimental liquid matrices such as plasma, serum, urine and tissue culture medium. The nitrite sensitivity depends on the matrix. The limit of detection is 2.5μM (125pmol) nitrite (in ultrapure, deionized distilled water).

Printed in USA. Revised 6/09.