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Griess Reagent System


Measures Nitrite, a Primary Breakdown Product of Nitric Oxide

  • Detects nitrate in a variety of types of biological samples


Catalog number selected: G2930

$ 193.00
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Griess Reagent System
1,000 assays
$ 193.00
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The Griess Reagent System measures nitrite (NO2), one of two primary stable and nonvolatile breakdown products of nitric oxide (NO). Nitric oxide is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. This assay relies on a diazotization reaction that was originally described by Griess in 1879. Through the years, many modifications to the original reaction have been described.

The Griess Reagent System is based on a chemical reaction that uses sulfanilamide and N-1-naphthylethylenediamine dihydrochloride (NED) under acidic (phosphoric acid) conditions. This system detects NO2 in a variety of biological and experimental liquid matrices such as plasma, serum, urine and tissue culture medium. The nitrite sensitivity is dependent on the matrix. The limit of detection is 2.5μM (125pmol) nitrite (in ultrapure, deionized, distilled water) using the protocol described in Technical Bulletin #TB229.

Original reference:
Griess, P. (1879) Chem. Ber. 12, 426–8.


What's in the box?

Item Part # Size

Sulfanilamide Solution

G294A 2 × 25ml

NED Solution

G295A 2 × 25ml

Nitrite Standard

G296A 1 × 1ml


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Download SDSPDF (92 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC Keep all solutions in their original light-protective plastic bottles.

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