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Alkaline Phosphatase, Calf Intestinal (CIAP) Protocol

Instructions for Use of Product(s)
M1821, M2825

Literature # 9PIM182

Calf intestinal alkaline phosphatase (CIAP) catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vehicle DNA by removing phosphate groups from both 5´-termini.

Revised 4/18.