Methylation Sensitivity of Isoschizomer Neoschizomer Pairs
Isoschizomers and neoschizomers often differ in their sensitivity to methylation. This can be a simple way to differentiate the methylation state of DNA. Iso/neoschizomer pairs sold by Promega are listed below.
|Methylated Sequence||Cleaved by||Not Cleaved by|
|m4CCGG||MspI (C/CGG)||HpaII (C/CGG)|
|Cm5CGG||MspI (C/CGG)||HpaII (C/CGG)|
|Cm4CGG||MspI (C/CGG)||HpaII (C/CGG)|
|CCm5CGGG||XmaI (C/CCGGG)||SmaI (CCC/GGG)|
|Gm6ATC||Sau3AI (/GATC)||MboI, NdeII (/GATC)|
|GATm5C||MboI, NdeII (/GATC)||Sau3AI (/GATC)|
|GATm4C||MboI (/GATC)||Sau3AI (/GATC)|
|GGTACm5C||KpnI (GGTAC/C)||Acc65I (G/GTACC)|
McClelland, M., Nelson, M. and Raschke, E. (1994) Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucl. Acids Res. 22, 3640.