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For ordering information on the products discussed here, please visit our Restriction Enzymes product listing. 

Isoschizomers and neoschizomers often differ in their sensitivity to methylation. This can be a simple way to differentiate the methylation state of DNA. Iso/neoschizomer pairs sold by Promega are listed below.

Methylated Sequence Cleaved by Not Cleaved by
m4CCGG MspI (C/CGG) HpaII (C/CGG)
Cm5CGG MspI (C/CGG) HpaII (C/CGG)
Cm4CGG MspI (C/CGG) HpaII (C/CGG)
CCm5CGGG XmaI (C/CCGGG) SmaI (CCC/GGG)
Gm6ATC Sau3AI (/GATC) MboI, NdeII (/GATC)
GATm5C MboI, NdeII (/GATC) Sau3AI (/GATC)
GATm4C MboI (/GATC) Sau3AI (/GATC)
GGTACm5C KpnI (GGTAC/C) Acc65I (G/GTACC)

Reference

McClelland, M., Nelson, M. and Raschke, E. (1994) Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucl. Acids Res. 22, 3640.