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Protein analysis

Protein Quantitation

Directly quantify cellular protein abundance using the bioluminescent HiBiT protein tagging system. The small 11 amino acid HiBiT peptide uses a streamlined, antibody-free protocol for detecting tagged proteins that requires only a luminometer for detection. Sensitive detection across a wide linear dynamic range detects proteins down to endogenous levels with the convenience of a single reagent addition. Multiple HiBiT detection solutions offer a universe of possibilities for researchers studying protein biology. Whether you chose to tag your protein by cloning or endogenously tag using CRISPR-Cas9 gene editing, the HiBiT system can label and detect cytoplasmic, secreted or surface protein expression. You can even detect HiBiT-labeled proteins on membranes—all without antibodies or extensive washing steps.

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Why Quantitate Protein Abundance?

Determining the abundance of a cellular protein is fundamental to understanding the cellular biology being studied. For cell-based assays, comparing the relative abundance between different experimental conditions is often the goal as opposed to measuring absolute protein levels. Many methods exist for quantitating cellular protein abundance, including colorimetric, fluorescent and bioluminescent assays. These approaches are often based on gel electrophoresis separation followed by staining or antibody-based western blotting. The ideal detection method is determined based on factors such as available antibodies, required quantitative dynamic range, throughput needs and protein expression level. The sensitivity of a method needs to be matched to the protein being detected. For example, proteins expressed at higher levels in cells can be measured using less sensitive colorimetric methods. However, for low-abundance proteins, bioluminescent methods that can quantitate down to nanomolar amounts may be necessary. By considering these factors, you can determine which experimental methods are best suited to quantitating cellular protein expression levels.

We offer a complete portfolio of protein analysis products supported by a growing library of educational resources, including technology guides, on-demand webinars, scientific articles and protocols.

Use the links below to learn more about protein analysis products, or to explore learning resources covering topics such as in vitro protein expression, protein purification methods and live-cell protein interaction analysis.