We believe this site might serve you best:

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Transcend™ Non-Radioactive Translation Detection Systems

Non-Radioactive Detection of In Vitro Translated Proteins

  • Biotin tag allows detection of 0.5–5ng of protein 3–4 hours after gel electrophoresis
  • No radioisotope handling, storage or disposal issues
  • Option of colorimetric or chemiluminescent detection

Choose a Detection Method

Size

Catalog number selected: L5070

$ 250.00 Your price: Log In

Transcend™ Non-Radioactive Translation Detection Systems
Colorimetric Detection/30 reactions
$ 250.00
Your price: Log In
Change Configuration

Non-radioactive detection of proteins synthesized in vitro. Using these systems, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [35S]methionine or other radioactive amino acids. Biotinylated lysine is added to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA complex (Transcend™ tRNA) rather than as a free amino acid. After SDS-PAGE and electroblotting, biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish Peroxidase (Streptavidin-HRP), followed either by colorimetric or chemiluminescent detection. Typically, these systems enable you to detect 0.5–5ng of protein in 3–4 hours after gel electrophoresis. This sensitivity is equivalent to that achieved with [35S]methionine incorporation and autoradiographic detection 6–12 hours after gel electrophoresis.

For more information, see the Protocols & Applications Guide.

Structure of Transcend™ tRNA.
Structure of Transcend™ tRNA.

Specifications

You are viewing: L5070 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Transcend™ tRNA

L506A 1 × 30μl View Product

Western Blue® Stabilized Substrate

S384C 1 × 35ml View Product

Streptavidin Alkaline Phosphatase

V559B 1 × 36μl View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: L5080 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Transcend™ Chemiluminescent Substrate A

L203A 1 × 25ml

Transcend™ Chemiluminescent Substrate B

L204A 1 × 25ml

Streptavidin HRP

L205A 1 × 15μl0.25mg/ml

Transcend™ tRNA

L506A 1 × 30μl View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom