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Transcend™ Non-Radioactive Translation Detection Systems

Non-Radioactive Detection of In Vitro Translated Proteins

  • Biotin tag allows detection of 0.5–5ng of protein 3–4 hours after gel electrophoresis
  • No radioisotope handling, storage or disposal issues
  • Option of colorimetric or chemiluminescent detection

Choose a Detection Method

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Catalog number selected: L5070

$ 250.00 Your price: Log In

Transcend™ Non-Radioactive Translation Detection Systems
Colorimetric Detection/30 reactions
$ 250.00
Your price: Log In
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Non-radioactive detection of proteins synthesized in vitro. Using these systems, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [35S]methionine or other radioactive amino acids. Biotinylated lysine is added to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA complex (Transcend™ tRNA) rather than as a free amino acid. After SDS-PAGE and electroblotting, biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish Peroxidase (Streptavidin-HRP), followed either by colorimetric or chemiluminescent detection. Typically, these systems enable you to detect 0.5–5ng of protein in 3–4 hours after gel electrophoresis. This sensitivity is equivalent to that achieved with [35S]methionine incorporation and autoradiographic detection 6–12 hours after gel electrophoresis.

For more information, see the Protocols & Applications Guide.

Structure of Transcend™ tRNA.
Structure of Transcend™ tRNA.

Protocols

Specifications

You are viewing: 30 reactions Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Transcend™ tRNA

L506A 1 x 30μl View Product

Western Blue® Stabilized Substrate

S384C 1 x 35ml View Product

Streptavidin Alkaline Phosphatase

V559B 1 x 36μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: 30 reactions Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Transcend™ Chemiluminescent Substrate A

L203A 1 x 25ml

Transcend™ Chemiluminescent Substrate B

L204A 1 x 25ml

Streptavidin HRP

L205A 1 x 15μl0.25mg/ml

Transcend™ tRNA

L506A 1 x 30μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

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