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Lumit™ FcRn Binding Immunoassay

Measure Neonatal Fc Receptor Binding

  • No wash or transfer: Simple add-mix-read protocol
  • Results in less than one hour
  • Scalable and high throughput: Perform assays in 96-well or 384-well plates

Size

Catalog number selected: W1151

$ 550.00
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This product is discontinued
Lumit™ FcRn Binding Immunoassay
100 assays
$ 550.00
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A Faster, Simpler FcRn Assay

The Lumit™ FcRn Binding Immunoassay is a novel homogeneous (no-wash) competition assay to measure the interaction between human FcRn and Fc proteins, including antibodies. In the assay, a human IgG1 labeled with LgBiT (Tracer-LgBiT) is used as the tracer. A C-terminal biotinylated human FcRn bound to Streptavidin-SmBiT (hFcRn-Biotin-SA-SmBiT) is used as the target. In the absence of an antibody analyte sample, Tracer-LgBiT binds to the hFcRn-SmBiT target, resulting in maximum luminescence signal. In analyte samples, unlabeled IgG will compete with Tracer-LgBiT for binding to the FcRn target, resulting in a concentration-dependent decrease in luminescent signal.

FcRn Function 
The neonatal Fc receptor (FcRn) is expressed in the endosomal compartments of a variety of cell types, including vascular endothelium and antigen-presenting cells (APCs). FcRn binds to the Fc region of immunoglobulin G (IgG) antibodies at acidic pH within endosomes. In utero, FcRn acts to transfer maternal IgG to the developing fetus. In adults, it is involved in recycling of IgG and albumin. Recycling by FcRn is the primary reason for the long half-life (several weeks) of IgG and albumin in serum. Furthermore, a critical factor for the success of therapeutic antibodies is their extended half-life, which contributes to better efficacy and long dosing schedule. Therefore, the FcRn-IgG interaction is a key parameter to optimize and track throughout the antibody drug development process.

Simple Protocol, Reproducible Results

The Lumit™ FcRn Binding Immunoassay is a solution-based homogeneous assay that requires no washes or sample transfer steps.

Lumit FcRn Binding Immunoassay workflow diagram.

How the Lumit™ FcRn Binding Immunoassay Works

In the absence of an antibody analyte, Tracer-LgBiT binds to the hFcRn-SmBiT target, resulting in maximum luminescence signal. In samples containing analyte, nonlabeled IgG will compete with Tracer-LgBiT for binding to the FcRn target, resulting in a concentration-dependent decrease in the luminescent signal.

Schematic for Lumit FcRn Binding Immunoassay 16519ma

Assay Performance Data

Lumit FcRn Binding Immunoassay detects IG-FcRn binding.

The Lumit™ FcRn Binding Immunoassay detects IgG-FcRn interaction. This is a representative standard curve of control antibody plotted as normalized data. Normalized luminescence is calculated by assigning 100% to the maximum bioluminescent signal obtained in absence of an analyte and then calculating percentage drop in signal in the presence of an analyte.

Lumit™ FcRn Binding Immunoassay can detect dose dependent, oxidation-based loss in antibody-FcRn affinity.

The Lumit™ FcRn Binding Immunoassay can detect dose-dependent, oxidation-based loss in antibody-FcRn affinity. Panitumumab was incubated with 0.3% H2O2 for 0–24 hours to induce methionine oxidation.

Specifications

You are viewing: W1151 Change Configuration

What's in the box?

Item Part # Size

Lumit™ Detection Substrate A

VB301D 1 × 75μl

hFcRn-Biotin

W109A 1 × 12μl

Streptavidin-SmBiT

W110A 1 × 12μl

Tracer-LgBiT

W111A 1 × 60μl

FcRn Assay Buffer

W112A 1 × 25ml

pH Adjustment Buffer

W113A 1 × 250μl

Control Antibody

W114A 1 × 120μl

SDS

Choose language:

Download SDSPDF (429 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

Specifications

You are viewing: W1152 Change Configuration

What's in the box?

Item Part # Size

Lumit™ Detection Substrate A

VB301D 10 × 75μl

hFcRn-Biotin

W109A 10 × 12μl

Streptavidin-SmBiT

W110A 10 × 12μl

Tracer-LgBiT

W111A 10 × 60μl

FcRn Assay Buffer

W112A 10 × 25ml

pH Adjustment Buffer

W113A 10 × 250μl

Control Antibody

W114A 10 × 120μl

SDS

Choose language:

Download SDSPDF (430 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

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