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HDAC-Glo™ I/II Assays and Screening System

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Detects HDAC Class I and II Enzyme Activity

  • Simple add-mix-measure protocol
  • Use purified enzymes, extracts or cells directly in culture plates
  • Screening System is no longer available

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Catalog number selected: G6420

$ 541.00
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HDAC-Glo™ I/II Assays and Screening System
Assay/10ml
$ 541.00
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Predictive, Flexible Assays for HDAC Activity

The HDAC-Glo™ I/II Assays and Screening Systems are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) class I and II enzymes using purified enzymes, extracts or cells directly in culture plates. The add-mix-measure protocol allows for simple measurement of deacetylating activities and easy implementation from benchtop to screening. The HDAC-Glo™ I/II Assays and Screening Systems can be used to determine HDAC inhibitor potency, selectively profile HDAC inhibitors, correlate HDAC inhibitor potency with cellular fate in same-well multiplexed viability assays or determine off-target HDAC effects of compounds.

The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a coupled enzymatic system in which a protease in the Developer Reagent cleaves the deacetylated peptide from the aminoluciferin substrate, releasing aminoluciferin, which is quantified in a reaction using Ultra-Glo™ recombinant firefly luciferase.

The assay reaction is typically complete within 15–45 minutes with no sample manipulation. The HDAC-mediated luminescent signal is persistent, with a half-life of greater than 3 hours, making the protocol amenable to automation in high-throughput formats and compatible with luminometers without injectors and allowing batch processing of multiwell plates. The HDAC assay is broadly useful for class I and II enzymes. The HDAC-Glo™ I/II Assays are highly sensitive, allowing you to obtain a dynamic range 10- to 100-fold higher than comparable fluorescence methods.

HDAC-Glo™ I/II Assay Chemistry
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 Number of Assays Per Plate

Product Cat.# # Assays Volume/Assay Plate Size
HDAC-Glo™ I/II Assay G6420 100 100µl 96-well
400 25µl 384-well
HDAC-Glo™ I/II Assay G6421 500 100µl 96-well
2,000 25µl 384-well
HDAC-Glo™ I/II Assay G6422 1,000 100µl 96-well
4,000 25µl 384-well

Trichostatin A

  • Known pan HDAC inhibitor that may be used as a positive control inhibitor

HeLa Nuclear Extract

  • May be used as a source of histone deacetylase activity
  • Diluted extract also can be used as an HDAC-Glo™ I/II Assay chemistry control

Specifications

You are viewing: G6420 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

HDAC-Glo™ I/II Buffer

G648A 1 × 25ml

HDAC-Glo™ I/II Substrate

G649A 1 × 1 vial

Developer Reagent

G653A 1 × 10μl

Trichostatin A

G656A 1 × 10μl 10mM View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

U.S. Pat. No. 8,632,992 and other patents pending.

Specifications

You are viewing: G6421 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

HDAC-Glo™ I/II Buffer

G648B 1 × 125ml

HDAC-Glo™ I/II Substrate

G649A 5 × 1 vial

Developer Reagent

G653B 1 × 50μl

Trichostatin A

G656A 1 × 10μl 10mM View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

U.S. Pat. No. 8,632,992 and other patents pending.

Specifications

You are viewing: G6422 Change Configuration

What's in the box?

Item Part # Size

HDAC-Glo™ I/II Buffer

G648B 2 × 125ml

HDAC-Glo™ I/II Substrate

G649B 1 × 1 vial

Developer Reagent

G653B 2 × 50μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

U.S. Pat. No. 8,632,992 and other patents pending.

Specifications

You are viewing: G6430 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

HDAC-Glo™ I/II Buffer

G648A 1 × 25ml

HDAC-Glo™ I/II Substrate

G649A 1 × 1 vial

Developer Reagent

G653A 1 × 10μl

Trichostatin A

G656A 1 × 10μl 10mM View Product

HeLa Nuclear Extract

G657A 1 × 10μl 5mg/ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for storage recommendations.

Specifications

You are viewing: G6431 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

HDAC-Glo™ I/II Buffer

G648B 1 × 125ml

HDAC-Glo™ I/II Substrate

G649A 5 × 1 vial

Developer Reagent

G653B 1 × 50μl

Trichostatin A

G656A 1 × 10μl 10mM View Product

HeLa Nuclear Extract

G657A 1 × 10μl 5mg/ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for storage recommendations.

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Petra

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