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GSH-Glo™ Glutathione Assay

V6911

A Luminescent-Based Assay for the Detection and Quantification of Glutathione (GSH)

  • Measure reduced and/or total glutathione levels in cells and tissues
  • Measure GSH levels directly from cell culture wells
  • Excellent sensitivity and easily scalable

Size

Catalog number selected: V6911

$ 395.00 Your price: Log In

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GSH-Glo™ Glutathione Assay
10ml
$ 395.00
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The GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples. A change in GSH levels is important in the assessment of toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay is based on the conversion of a luciferin derivative into luciferin in the presence of GSH. The reaction is catalyzed by a glutathione S-transferase (GST) enzyme supplied in the kit. The luciferin formed is detected in a coupled reaction using Ultra-Glo™ Recombinant Luciferase that generates a glow type luminescence that is proportional to the amount of glutathione present in cells. The assay provides a simple, fast and sensitive alternative to colorimetric and fluorescent methods and can be adapted easily to high-throughput applications.

The GSH-Glo Glutathione Assay

6956MA-W

The assay is performed in two steps. In the 1st step, cells are lysed in the presence of the luciferin-NT substrate and glutathione S-transferase. Glutathione in the cells drives the formation of luciferin. In the 2nd step, Luciferin Detection Reagent is added to produce light that is directly proportional to the amount of GSH in the reaction.

GSH Depletion and Recovery in Treated Cells

7330MA-W

HeLa cells (5000 cells/well in 96 well format) were exposed to l-Buthionine-sulfoximine (BSO). BSO inhibits GSH synthesis thus reducing cellular GSH level.

20hr BSO: Cells treated with BSO and assayed for GSH after 20 hours.

BSO + Recovery: Cells treated with BSO for 20 hours then washed 2X with PBS and covered with fresh media without BSO. Cells assayed for GSH after 40 hours.

40hr BSO: Cells treated with BSO, washed at 20 hours and fresh media plus BSO added. Cells assayed for GSH after 40 hours.

The simple two-reagent-addition assay minimizes the number of assay steps compared to conventional GSH assays and is adapted easily to higher throughput applications. No deproteination step is required, and results are achieved in as quickly as 30 minutes. 

The luminescent method avoids inherent background fluorescence associated with other methods thereby providing excellent signal-to-background ratios. A half-life greater than 5 hours results in a stable signal as well. 

GSH Depletion by Acetaminophen in Hepatocytes

7364MA-W

GSH levels in lysates from adherent cells in 24 well plates were measured with the GSH-Glo Glutathione Assay. GSH concentrations were determined by interpolation from a GSH standard curve generated using the bioluminescent system. Exposure to 5mM acetaminophen for three hours substantially reduced GSH only after treatment for two days with a P450 inducer, 30 μM pregnenolone 16a-carbonitrile (PCN).

Protocols

Specifications

You are viewing: 10ml Change Configuration

What's in the box?

Item Part # Size

Reconstitution Buffer with esterase

V144A 1 x 10ml

Luciferin-NT

V687A 1 x 100μl

GSH-Glo™ Reaction Buffer

V688A 1 x 10ml

Glutathione S-Transferase

V689A 1 x 100μl

Glutathione, 5mM

V690A 1 x 100μl

Luciferin Detection Reagent

V859A 1 x 1 each

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: 50ml Change Configuration

What's in the box?

Item Part # Size

Reconstitution Buffer with esterase

V144B 1 x 50ml

Luciferin-NT

V687B 1 x 500μl

GSH-Glo™ Reaction Buffer

V688B 1 x 50ml

Glutathione S-Transferase

V689B 1 x 500μl

Glutathione, 5mM

V690A 1 x 100μl

Luciferin Detection Reagent

V859B 1 x 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Certain applications of this product may require licenses from others.

Resources

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