The PureYield™ Plasmid Miniprep System isolates up to 15μg of transfection-quality plasmid DNA from 600μl to 3ml of bacterial culture in 10 minutes. The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the performance in applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method.
The system can be used with centrifugation or vacuum-based protocols, and includes a convenient lysis/neutralization indicator dye.