Notes: Delta-like 1 homolog (DLK1) functions in cell differentiation during development in both a Notch-dependent and -independent manner. Here, the CheckMate™/Flexi® Mammalian Two-Hybrid System and Dual-Luciferase® Reporter Assay System are used to monitor DLK1-DLK1, DLK1-fibronectin, and DLK1-cysteine-rich FGF receptor interactions. This further illustrates the function of the Notch-independent mechanism in development. (5104)

Notes: Chickens were immunized with PT-gliadin and splenocytes were isolated. Anti-PT-gliadin-positive cells were enriched on magnetic beads coated with PT-gliadin. Total RNA was extracted from the bead-bound cells with the ReliaPrep™ RNA Cell Miniprep System and used to clone the chicken IgY hypervariable regions. The scFv expression vectors were purified with the PureYield™ Plasmid Miniprep or Midiprep System prior to transformation into BL21(DE3) cells. ELISA assays were developed with standard TMB substrate and read with the GloMax® Instrument. (4618)