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Int J Biol Macromol. 97, 460–7. The non-canonical NOTCH1 ligand Delta-like 1 homolog (DLK1) self interacts in mammals. 2017

Traustadóttir, G.Á., Jensen, C.H., Garcia Ramirez, J.J., Beck, H.C., Sheikh, S.P., Andersen, D.C.

Notes: Delta-like 1 homolog (DLK1) functions in cell differentiation during development in both a Notch-dependent and -independent manner. Here, the CheckMate™/Flexi® Mammalian Two-Hybrid System and Dual-Luciferase® Reporter Assay System are used to monitor DLK1-DLK1, DLK1-fibronectin, and DLK1-cysteine-rich FGF receptor interactions. This further illustrates the function of the Notch-independent mechanism in development. (5104)

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BMC Biotechnol. 15, 109. Novel avian single-chain fragment variable (scFv) target dietary gluten and related natural grain prolamins, toxic entities of celiac disease. 2015

Stadlmann, V., Harant, H., Korschineck, I., Hermann, M., Forster, F. and Missbichler, A.

Notes: Chickens were immunized with PT-gliadin and splenocytes were isolated. Anti-PT-gliadin-positive cells were enriched on magnetic beads coated with PT-gliadin. Total RNA was extracted from the bead-bound cells with the ReliaPrep™ RNA Cell Miniprep System and used to clone the chicken IgY hypervariable regions. The scFv expression vectors were purified with the PureYield™ Plasmid Miniprep or Midiprep System prior to transformation into BL21(DE3) cells. ELISA assays were developed with standard TMB substrate and read with the GloMax® Instrument. (4618)

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