We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

XpressAmp™ Direct Amplification Reagents, Custom

Rapid, Extraction-Free Viral Sample Preparation for RT-qPCR Amplification

  • Ten-minute, room-temperature sample lysis
  • Easily automatable for high-throughput needs
  • Use Restrictions: For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Please Enquire
This product is discontinued
XpressAmp™ Direct Amplification Reagents, Custom

Direct Amplification for Rapid PCR Results

The XpressAmp™ Direct Amplification Reagents, Custom, provide a fast, RNA extraction-free method to prepare viral samples for PCR-based amplification using commonly available RT-qPCR reagents. Collect the samples by nasopharyngeal swab in universal or viral transport media, and perform direct amplification analysis in RT-qPCR. The simple sample preparation method requires only a 10-minute, room-temperature incubation that is easy to automate.

How the XpressAmp™ Direct Amplification Reagents, Custom, Work

Collect and Store Sample

Collect and Store Sample
Use commonly available nasopharyngeal swabs stored in transport media.

Lyse Sample

Lyse Sample
Mix sample and XpressAmp™ Lysis Buffer (1:1). Incubate for 10 minutes at room temperature.

Amplify and Analyze

Amplify and Analyze
Add lysed sample to RT-qPCR amplification containing XpressAmp™ Solution.

Viral RNA Detection Without the Need for Extraction

XpressAmp™ Detection of RNA from Inactivated Influenza/RSV Virus Pellet

XpressAmp™ Detection of RNA from Inactivated Influenza/RSV Virus Pellet-User 1
XpressAmp™ Detection of RNA from Inactivated Influenza/RSV Virus Pellet-User 2

Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with RSV A and Influenza B (Hong Kong) virus reconstituted from Helix Elite™ Inactivated Standard, Inactivated Influenza A/B and Respiratory Syncytial Virus. This high-level virus sample (1 × 103 copies/μl) was diluted 1:10 and 1:100 in VTM to create the medium- and low-level virus samples. In parallel, two users created sample lysates from the spiked VTM samples using the XpressAmp™ Direct Amplification Reagents, Custom. Both users then detected the presence of RSV A and Influenza B by RT-qPCR using GoTaq® 1-Step Probe qPCR System (Cat.# A6121) supplemented with the XpressAmp™ Solution. N=6.

Amplification of Synthetic SARS-CoV-2 RNA from XpressAmp™ Lysates

Amplification of Synthetic SARS-CoV-2 RNA from XpressAmp™ Lysates

Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with Synthetic SARS-CoV-2 RNA Control 2 (Twist Biosciences, Cat.# 102024, final concentration 1 × 104 copies/μl). Spiked VTM samples were lysed by combining 5μl of sample with 5μl of prepared XpressAmp™ Lysis Buffer and incubated at room temperature for 10 minutes. Following incubation, 5μl of sample lysate was added to a monoplex GoTaq® Probe 1-Step RT-qPCR (25μl) containing XpressAmp™ Solution and amplified using the 2019 nCoV RUO kit (IDT, Cat.# 10006713) and thermal cycled according to the CDC protocol. N=8 amplification replicates.

Compatible for Use with GoTaq® Probe RT-qPCR System

Combine XpressAmp™ Direct Amplification Reagents with GoTaq® Probe RT-qPCR System to rapidly detect viral RNA using standard or fast cycling on most real-time PCR instruments.

Protocol Available Upon Request

Request a protocol for this product.

Request Protocol

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-manos

Manos

UK