GoTaq® MDx Hot Start Polymerase
Inhibits Polymerase Activity at Temperatures below 70°C
- High-concentration and glycerol-free formulations also available
- All formulations supplied at ≥5u/μl, except for high-concentration formulation, which is supplied at ≥50u/μl
- Custom formats and formulations
- Manufactured under cGMP for consistent, lot-to-lot performance
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated.
Catalog Number:
Choose an option
Size
Catalog Number: D6001
Catalog Number: D6005
Catalog Number: D6006
Catalog Number: D6008
Catalog Number: D6101
Catalog Number: D6201
Consistent and Robust Amplification
GoTaq® MDx Hot Start Polymerase contains GoTaq® MDx DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94–95°C for two minutes. This allows hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets because primer-dimer and secondary products are eliminated or minimized. In some cases, hot-start PCR may improve yields. The glycerol-free product formulation is further purified to remove glycerol, making the enzyme suitable for further manufacture processing and lyophilization.
- Manufactured under cGMP using validated equipment, processes and method under a certified Quality System to ensure consistent product performance lot-to-lot.
- QC-tested for bacterial, fungal and mammalian DNA to ensure low DNA contamination.
- Flexible product formats and custom formulations available.
GoTaq® MDx Hot Start Polymerase is a general purpose reagent intended for general laboratory use. It is suitable for use as a component of molecular diagnostic assays, where applicable country laws allow. Separate license or royalty payments not required. This product alone does not provide any diagnostic result.
PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.
That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.
Consistent Performance
Variance of 12 separately manufactured lots of product from manufacture target concentration
GoTaq® MDx DNA Polymerase products are manufactured under cGMP using validated equipment, processes and methods under a certified Quality System to ensure consistent product performance lot-to-lot.
Reduce nonspecific amplification with hot-start PCR using GoTaq® MDx Hot Start Polymerase
High Quality
| GoTaq® MDx DNA Polymerase products meet stringent specifications for: | |
|---|---|
| Purity | ≥90% (Reverse Phase- HPLC) |
| DNA Contamination | One unit contains less than:
|
| Nuclease Contamination | Undetectable exonuclease, endonuclease, nicking and ribonuclease activity |
| Performance | Quantitative amplification of DNA target. Amplification of hot-start model template produces a single 1.5kb band without extraneous bands (GoTaq® MDx Hot Start Polymerase formulation only) |
| Stability | Stable for 24 months from date of manufacture |
Customization
Rely on an Expert Partner
Choose a trusted partner with the robust and reliable reagents you need for your molecular assay and the experience to support you throughout your assay development process.
More than 30 years of manufacturing experience
Commitment to high-quality standards
- ISO 13485 & ISO 9001 certified
- cGMP-manufacturing capabilities
Consistent and reliable amplification products
Flexible, custom, made-to-order solutions available
To use GoTaq® MDx Hot Start Polymerase in a custom format or to distribute GoTaq® MDx Hot Start Polymerase, contact the Promega Custom Order Department to discuss specific requirements.
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 1 × 750μl | 25mM | View Product |
|
GoTaq® MDx Hot Start Polymerase |
D600A | 1 × 100u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 1 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 1 × 1ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 3 × 750μl | 25mM | View Product |
|
GoTaq® MDx Hot Start Polymerase |
D600B | 1 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 4 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 4 × 1ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 15 × 750μl | 25mM | View Product |
|
GoTaq® MDx Hot Start Polymerase |
D600B | 5 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 20 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 20 × 1ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 60 × 750μl | 25mM | View Product |
|
GoTaq® MDx Hot Start Polymerase |
D600B | 20 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 80 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 80 × 1ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 2 × 1.2ml | 25mM | View Product |
GoTaq® MDx Hot Start Polymerase |
D610A | 1 × 1,000u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890C | 2 × 5ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 3 × 750μl | 25mM | View Product |
GoTaq® MDx Hot Start Polymerase, Glycerol-Free |
D620A | 1 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 4 × 1ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
Resources
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