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NanoBRET® Target Engagement K192 Kinase Selectivity System

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Profile Selectivity Across a Diverse Kinase Panel in Live Cells

  • Profile compound cellular selectivity against a panel of 192 human kinases, broadly representing the kinome
  • Optimized, addition-only cellular protocol provides quantitative compound occupancy data for 192 full-length kinases in a single experiment
  • K192 System provides the transfection-ready, arrayed K192 Kinase Vector Panel and a single NanoBRET® TE Kinase Assay

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Catalog number selected: NP4050

$ 22,700.00
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NanoBRET® Target Engagement K192 Kinase Selectivity System
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$ 22,700.00
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Profile Compound Selectivity Against 192 Kinases in Live Cells

The NanoBRET® Target Engagement (TE) K192 Kinase Selectivity System can quantitatively measure intracellular compound occupancy against a panel of 192 kinases in a single experiment. The system relies on the NanoBRET® TE technology, a bioluminescence resonance energy transfer (BRET) method that uses the small, extremely bright NanoLuc® luciferase as the energy donor and a cell-permeable NanoBRET® TE Tracer as the energy acceptor. The K192 Kinase Selectivity System is a live-cell method using an addition-only, multiwell plate format with a single NanoBRET® TE Kinase Assay. The NanoBRET® TE K192 Kinase Selectivity System is optimized for each kinase to provide quantitative occupancy data, enabling cellular profiling of compound-kinase interactions.

NanoBRET® Target Engagement Assay Principle

The NanoBRET® TE K192 Kinase Selectivity System uses the NanoBRET® TE technology, a bioluminescence resonance energy transfer (BRET) method. In this method, a target is expressed in mammalian cells as a target-NanoLuc® fusion protein. BRET is achieved by luminescent energy transfer from NanoLuc® luciferase to a cell-permeable NanoBRET® TE Tracer that is bound to the target-NanoLuc® fusion protein. A test compound that is cell-permeable and competes with the tracer for target binding will result in a loss of NanoBRET® signal. Using the optimized assay conditions provided for each target, the assay yields quantitative intracellular compound affinity and occupancy. Compound occupancy is quantified using controls that allow the BRET signal to be normalized.

NanoBRET™ Target Engagement Assay Principle

K192 Kinase Selectivity System Provides Turn-Key Cellular Profiling

The NanoBRET® TE K192 Kinase Selectivity System contains a NanoBRET® TE K192 Kinase Vector Panel and a single NanoBRET® TE Intracellular Kinase Assay (K-10). The optimized protocol offers an addition-only workflow using multiwell plates. Begin by simply transfecting the vector panel into HEK293 cells or TransfectNow™ HEK293 Cells in a multiwell plate. Following overnight incubation, add your test compounds to the assay plate and perform the NanoBRET® TE Kinase Assay (K-10), according to the optimized protocol provided. A mutimode plate reader is used to measure BRET.

NanoBRET™ TE K192 Kinase Selectivity System Complete Workflow

K192 Kinase Vector Panel Simplifies Cellular Kinase Selectivity Profiling


  • The NanoBRET® TE K192 Kinase Vector Panel contains 192 kinase-NanoLuc® fusion vectors that are broadly spread across the kinome (green dots on dendrogram).
  • The NanoBRET® TE K192 Kinase Vector Panel is supplied in two 96-well plates (A and B).
  • Each well of the K192 Kinase Vector Panel has a unique mix of two vectors—one kinase-NanoLuc® fusion vector and either carrier DNA or a regulatory protein expression vector for optimal transfections.
  • The addition-only microplate workflow uses a pre-arrayed transfection-ready Kinase Vector Panel and a single NanoBRET® TE Kinase Assay.
K192 Kinase Vector Panel

Diverse Panel of 192 Kinases to Represent the Kinome

The 192 Kinases in the NanoBRET® TE K192 Kinase Selectivity System are represented as green dots in the dendrogram, showing that the system broadly covers the kinome.

Kinases Present in the K192 Panel
Table of Kinases Present in the K192 Panel

Note: A name with a forward slash "/" indicates the kinase and the regulatory genes are both present in the vector mix and will be co-expressed when transfected. For example, “CDK/CCNB1” in the table indicates CDK1 is co-expressed with CCNB1.

Dendrogram showing that the K192 Panel broadly covers the kinome

Get The Live-Cell Kinase Selectivity Profiling Data You Need

Cell-free biochemical techniques for measuring binding or activity of kinase inhibitors can fail to accurately predict engagement of kinases inside a cell.

The dendrograms compare the target occupancy obtained with 1µM crizotinib using the live-cell NanoBRET® TE K192 Kinase Selectivity System versus a biochemical cell-free approach. The results showed an improved spectrum of activity in cells, with only 16 targets engaged by crizotinib at 50% occupancy or greater using the NanoBRET® TE method, compared to 49 kinases engaged using the biochemical approach.

The 16 kinases that bound with >50% occupancy using the K192 Kinase Selectivity System are tabularized along with the mean occupancy obtained from three independent experiments. The standard deviations for these measurements indicates that the K192 Kinase Selectivity System is reproducible.

NanoBRET® TE Cellular
Crizotinib, 16 hits

Dendrogram showing target occupancy obtained with 1µm of crizotinib using the live-cell NanoBRET™ TE K192 Kinase Selectivity System

Biochemical
Crizotinib, 49 hits

Dendrogram showing the target occupancy obtained with 1µm of crizotinib using the biochemical cell-free approach
NanoBRET® TE K192 Kinase Selectivity Results for 1µM Crizotinib
NanoBRET™ TE K192 Kinase Selectivity Results for 1µM Crizotinib
cellchemicalbiologypaper

The Cell Chemical Biology publication is the original demonstration of how NanoBRET® TE could be used for broad kinase selectivity profiling in live cells. This publication illustrates the first live-cell method to quantitatively profile compound occupancy against hundreds of kinases.

Read the Paper
starprotocolspaper

The STAR Protocols publication improves upon the Cell Chemical Biology work and is the basis for the NanoBRET® TE K192 Kinase Selectivity System. The authors show how a single NanoBRET® TE Kinase Assay can be used to profile compound engagement across 192 full-length kinases in a single live-cell experiment using a simple workflow.

Read the Paper

Specifications

You are viewing: NP4050 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

NanoBRET® Nano-Glo® Substrate

N157D 1 × 3.3ml View Product

Tracer Dilution Buffer

N219B 1 × 50ml View Product

Extracellular NanoLuc® Inhibitor

N235C 1 × 1.1ml View Product

NanoBRET® Tracer K-10

N264B 10 × 300μl 400μM

NanoBRET® TE K192 Vector Plate A

NP401A 2 × 1 each

NanoBRET® TE K192 Vector Plate B

NP402A 2 × 1 each

Adhesive Foil Plate Seals

NP4000 1 × 1 each

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

NanoBRET® TE Selectivity Vector Panel Limited Use Label License

BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.

User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.

In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

NanoBRET® TE Tracers V2 Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(a) use NanoLuc®-branded luciferase or derivatives, or subunits of the NanoBiT® technology for all energy transfer determinations conducted with this product; and
(b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(c) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.

In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

NanoBRET® TE LAR Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 10,067,149 and 10,024,862, European Pat. No. 2932267, Japanese Pat. Nos. 6751294 and 7092691 and other patents and patents pending.

U.S. Pat. No. 10,513,694, European Pat. No. 3313846 and other patents.

Licensed from Kazusa Genome Technologies.

Specifications

You are viewing: NP4060 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

NanoBRET® Nano-Glo® Substrate

N157D 1 × 3.3ml View Product

Tracer Dilution Buffer

N219B 1 × 50ml View Product

Extracellular NanoLuc® Inhibitor

N235C 1 × 1.1ml View Product

NanoBRET® Tracer K-10

N264B 10 × 300μl 400μM

Transfection Carrier DNA

E488B 1 × 100μg View Product
pNL1.1.CMV[Nluc/CMV] Vector N109A 1 × 20μg View Product

NanoLuc®-HIPK2 Fusion Vector

NV322A 1 × 20μg 1μg/μl View Product

NanoBRET® TE K192 Vector Plate A

NP401A 2 × 1 each

NanoBRET® TE K192 Vector Plate B

NP402A 2 × 1 each

Adhesive Foil Plate Seals

NP4000 1 × 1 each

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

NanoBRET® TE Selectivity Vector Panel Limited Use Label License

BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.

User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.

In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

NanoBRET® TE Tracers V2 Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(a) use NanoLuc®-branded luciferase or derivatives, or subunits of the NanoBiT® technology for all energy transfer determinations conducted with this product; and
(b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(c) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.

In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

NanoBRET® TE LAR Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

NanoLuc® ORF TE Vector Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. No other use of this product is authorized without the prior express written consent of Promega.

In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product (NanoLuc® ORF Vector) for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(2a) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; and
(2b) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 10,067,149 and 10,024,862, European Pat. No. 2932267, Japanese Pat. Nos. 6751294 and 7092691 and other patents and patents pending.

U.S. Pat. No. 10,513,694, European Pat. No. 3313846 and other patents.

Licensed from Kazusa Genome Technologies.

Specifications

You are viewing: NP4100 Change Configuration

What's in the box?

Item Part # Size

NanoBRET® TE K192 Vector Plate A

NP401A 2 × 1 each

NanoBRET® TE K192 Vector Plate B

NP402A 2 × 1 each

Adhesive Foil Plate Seals

NP4000 1 × 1 each

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

NanoBRET® TE Selectivity Vector Panel Limited Use Label License

BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.

User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.

In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

Licensed from Kazusa Genome Technologies.

Specifications

You are viewing: NP4101 Change Configuration

What's in the box?

Item Part # Size

NanoBRET® TE K192 Vector Plate A

NP401A 1 × 1 each

NanoBRET® TE K192 Vector Plate B

NP402A 1 × 1 each

Adhesive Foil Plate Seals

NP4000 1 × 1 each

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

NanoBRET® TE Selectivity Vector Panel Limited Use Label License

BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.

User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.

In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.

For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.

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