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CellTiter-Glo® 2.0 Cell Viability Assay

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A Luminescent Cell Viability Assay for Fast, Easy, Everyday Use

  • ATP assay for detection of viable cells
  • Reduced preparation time and convenient repeat use over multiple days
  • Same great performance as classic CellTiter-Glo® Cell Viability Assay
  • Integrates with the MyGlo™ Reagent Reader

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$ 137.00
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CellTiter-Glo® 2.0 Cell Viability Assay
CellTiter-Glo® 2.0 Assay/10ml
$ 137.00
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11542MD-W

CellTiter-Glo® 2.0 Measures ATP, a Key Indicator of Cell Health

CellTiter-Glo® 2.0, based on the original CellTiter-Glo® Cell Viability Assay chemistry, is a single-reagent assay with improved stability for convenient storage and simpler setup. Whether you perform cell proliferation assays in a single plate or process hundreds of plates at a time, the ready-to-use reagent is designed for fast and easy everyday use. A highly sensitive ATP assay with broad linearity, it is amenable to high- throughput screening and multiplex applications.

The CellTiter-Glo® 2.0 Assay determines the number of viable cells in culture by quantifying ATP, which indicates the presence of metabolically active cells. Luminescence readout is directly proportional to the number of viable cells in culture.

CellTiter-Glo-luminescent-atp-assay

Simple Assay Setup and Enhanced Stability

A single, ready-to-use reagent and simple "add-mix-measure" protocol mean there's no need to mix components.

The CellTiter-Glo® 2.0 reagent is stable at room temperature and at 4°C, allowing fast and easy assay implementation, and eliminating the thawing and mixing required with other ATP assays. Enhanced stability also allows convenient storage.

Percent Activity Remaining CellTiter-Glo® 2.0 Reagent Stability CellTiter-Glo® "Original" Reagent Stability
>85% Activity at 22°C 7 days 12 hours
>85% Activity at 4°C 2 months 3.5 days
CellTiter-Glo 2.0 cell viability assay protocol

Assay Performance

The CellTiter-Glo® 2.0 Cell Viability Assay exhibits the same high performance as the classic CellTiter-Glo® Assay, making it suitable for use from benchtop scale to high-throughput screening.

cell proliferation assay sensitivity 96-well 11815MC-W-a
cell proliferation assay sensitivity 384 well 11815MC-W-b
cell proliferation assay sensitivity 1536 well 11815MC-W-c

High sensitivity and broad linearity. Luminescence measured with the CellTiter-Glo® 2.0 Assay is proportional to the number of viable Jurkat cells in culture over three orders of magnitude in 96-well, 384-well and 1536-well plates.


Line graph showing that the high sensitivity of the CellTiter-Glo® 2.0 Assay system enables reliable performance even from low cell densities.

High sensitivity of the assay system enables reliable performance even from low cell densities. Jurkat cells were seeded in all-white 96-well plates at 400, 2,000 and 10,000 cells/well and were treated with Bortezomib for 20 hours. Viability was measured with CellTiter-Glo® 2.0 and MyGlo™ Reagent Reader

Multiplex With Other Cell Viability or Cytotoxicity Assays

CellTiter-Glo® 2.0 cell viability assay can be multiplexed with other assays, reducing cell culture costs and labor. Multiplexing assays in the same sample well allows complementary measures of cell health to correct for errors in culturing, plating or assay chemistry interferences.

In the example shown here, CellTox™ Green cytotoxicity assay reagent was added to bortezemib-treated K562 cells after 48 hours of exposure. Fluorescence associated with cytotoxicity was measured. The CellTiter-Glo® 2.0 assay reagent was added and luminescence measured as an indicator of cell proliferation.

example cell viability and cytotoxicity data 12023MB-W

Bioluminescence Resource Center

Learn more about how luminescence works, what makes it different from fluorescence and how you can use luminescence to fuel your own research.

Specifications

You are viewing: G9241 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® 2.0 Reagent

G924A 1 × 10ml

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

Patent Pending.

Specifications

You are viewing: G9242 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® 2.0 Reagent

G924B 1 × 100ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

Patent Pending.

Specifications

You are viewing: G9243 Change Configuration

What's in the box?

Item Part # Size

CellTiter-Glo® 2.0 Reagent

G924C 1 × 500ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

GG See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

Patent Pending.

Specifications

You are viewing: MG1010 Change Configuration

What's in the box?

Item Part # Size

MyGlo™ Reagent Reader

  • USB Cable
  • Wall Power Adapter
  • Plug Adapters
MG1000 1 × 1 each

CellTiter-Glo® 2.0 Reagent

G9241 1 × 10ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

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Luca

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