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TnT® T7 Insect Cell Extract Protein Expression System

Single-Tube, Coupled Transcription/Translation for Cell-Free Protein Expression using Insect Cell Extract

  • Quick, single-tube, coupled transcription/translation system for the cell-free expression of proteins from DNA templates
  • Extract derived from Spodoptera frugiperda Sf21 cell line
  • Proteins expressed from genes cloned downstream of the T7 promoter; companion vectors available

Size

Catalog number selected: L1101

$ 192.00 Your price: Log In

TnT® T7 Insect Cell Extract Protein Expression System
10 reactions
$ 192.00
Your price: Log In
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The TnT® T7 Insect Cell Extract Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system for the cell-free expression of proteins using an extract derived from the Spodoptera frugiperda Sf21 cell line. Protein synthesis reactions are initiated by the addition of a DNA template, eliminating the need for the time-consuming process of in vitro RNA synthesis.

All components necessary for the transcription/translation are present in the TnT® T7 ICE Master Mix. To initiate protein synthesis, the only component that must be added is the DNA template. Reactions are complete within 4 hours.

Proteins are expressed from genes cloned downstream of the T7 promoter. Companion vectors have been designed to achieve optimal yield with this system (pF25A and pF25K). They contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency. Using the TnT® T7 Insect Cell Extract Protein Expression System and these vectors, 75μg/ml of functional protein can be produced.

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

GenBank®/EMBL Accession Numbers and Sequence Information

pF25A ICE T7 Flexi® Vector Accession Number EU754721; pF25A ICE T7 Flexi® Vector Vector sequence text file.
pF25K ICE T7 Flexi® Vector Accession Number  EU754722; pF25K ICE T7 Flexi® Vector Vector sequence text file.

9695MD_500

Schematic of protein expression with TnT® T7 Insect Cell Extract System.

Typical protein yields using TNT T7 Insect Cell Extract Protein Expression System.

Typical protein yields using the TnT® T7 Insect Cell Extract Protein Expression System.

Protocols

Specifications

You are viewing: L1101 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Luciferase ICE T7 Control DNA

L105B 1 × 10μg

TnT® T7 ICE Master Mix

L110A 1 × 400μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

Ezure, T., Suzuki, T., Higashide, S., Shintani, E., Endo, K., Kobayashi, S., Shikata, M., Ito, M., Tanimizu, K., Nishimura, O. (2006) Cell-free protein synthesis system prepared from insect cells by freeze-thawing. Biotechnol. Prog. 22, 1570–7.

Use of this vector for applications outside of the TNT® T7 Insect Cell Extract Protein Expression System may require a license from Shimadzu Corporation. For more information contact: t-direct@shimadzu-biotech.jp

Specifications

You are viewing: L1102 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Luciferase ICE T7 Control DNA

L105B 1 × 10μg

TnT® T7 ICE Master Mix

L110A 4 × 400μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

Ezure, T., Suzuki, T., Higashide, S., Shintani, E., Endo, K., Kobayashi, S., Shikata, M., Ito, M., Tanimizu, K., Nishimura, O. (2006) Cell-free protein synthesis system prepared from insect cells by freeze-thawing. Biotechnol. Prog. 22, 1570–7.

Use of this vector for applications outside of the TNT® T7 Insect Cell Extract Protein Expression System may require a license from Shimadzu Corporation. For more information contact: t-direct@shimadzu-biotech.jp

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