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Direct PCR: XpressAmp™ Direct Amplification Reagents

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Rapid, Extraction-Free Viral Sample Preparation for RT-qPCR Amplification

  • Ten-minute, room-temperature sample lysis
  • Easily automatable for high-throughput needs
  • Use Restrictions: For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

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Catalog number selected: A8882

$ 500.00
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Direct PCR: XpressAmp™ Direct Amplification Reagents
250 reactions
$ 500.00
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Direct Amplification for Rapid PCR Results

The XpressAmp™ Direct Amplification Reagents provide a fast, RNA extraction-free method to prepare viral samples for PCR-based amplification using commonly available RT-qPCR reagents. Collect the samples by nasopharyngeal swab in universal or viral transport media, and perform direct amplification analysis in RT-qPCR. The simple sample preparation method requires only a 10-minute, room-temperature incubation that is easy to automate.

How the XpressAmp™ Direct Amplification Reagents Work

Direct PCR workflow Step 1: Collect and Store Sample

Collect and Store Sample
Use commonly available nasopharyngeal swabs stored in transport media.

DIrect PCR workflow Step 2: Lyse Sample

Lyse Sample
Mix sample and XpressAmp™ Lysis Buffer (1:1). Incubate for 10 minutes at room temperature.

Direct PCR workflow Step 3: Amplify and Analyze

Amplify and Analyze
Add lysed sample to RT-qPCR amplification containing XpressAmp™ Solution.

Direct PCR Amplification Benefits and Challenges

Viral RNA Detection Without the Need for Extraction

XpressAmp™ Detection of RNA from Inactivated Influenza/RSV Virus Pellet

XpressAmp Detection of RNA from Inactivated Influenza/RSV Virus Pellet
XpressAmp Detection of RNA from Inactivated Influenza/RSV Virus Pellet

Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with RSV A and Influenza B (Hong Kong) virus reconstituted from Helix Elite™ Inactivated Standard, Inactivated Influenza A/B and Respiratory Syncytial Virus. This high-level virus sample (1 × 103 copies/μl) was diluted 1:10 and 1:100 in VTM to create the medium- and low-level virus samples. In parallel, two users created sample lysates from the spiked VTM samples using the XpressAmp™ Direct Amplification Reagents. Both users then detected the presence of RSV A and Influenza B by RT-qPCR using GoTaq® Probe 1-Step qPCR System (Cat.# A6121) supplemented with the XpressAmp™ Solution. N=6.

Amplification of Synthetic SARS-CoV-2 RNA from XpressAmp™ Lysates

Amplification of Synthetic SARS-CoV-2 RNA from XpressAmp™ Lysates

Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with Synthetic SARS-CoV-2 RNA Control 2 (Twist Biosciences, Cat.# 102024, final concentration 1 × 104 copies/μl). Spiked VTM samples were lysed by combining 5μl of sample with 5μl of prepared XpressAmp™ Lysis Buffer and incubated at room temperature for 10 minutes. Following incubation, 5μl of sample lysate was added to a monoplex GoTaq® Probe 1-Step RT-qPCR (25μl) containing XpressAmp™ Solution and amplified using the 2019 nCoV RUO kit (IDT, Cat.# 10006713) and thermal cycled according to the CDC protocol. N=8 amplification replicates.

Saliva Direct Protocol for SARS-CoV-2 Surveillance Testing

Inactivation of SARS-CoV-2 Virus by XpressAmp™ Lysis Buffer

An independent laboratory performed an evaluation of the ability of the XpressAmp™ Lysis Buffer, used to prepare viral sample for RT-qPCR analysis, to inactivate the SARS-CoV-2 virus.

SARS-CoV-2 Viral Inactivation White Paper

SARS-CoV-2 Viral Inactivation by Xpressamp Reagents
Read PDF

Evidence of Viral Inactivation by XpressAmp™ Lysis Buffer Video

Specifications

You are viewing: A8882 Change Configuration

What's in the box?

Item Part # Size

XpressAmp™ Lysis Buffer

A886A 1 × 15ml

XpressAmp™ Solution

A887A 1 × 1.5ml
1-Thioglycerol A208A 2 × 75μl

SDS

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Download SDSPDF (279 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

CC

Specifications

You are viewing: A8880 Change Configuration

What's in the box?

Item Part # Size

XpressAmp™ Lysis Buffer

A886B 1 × 200ml

XpressAmp™ Solution

A887B 1 × 18ml

1-Thioglycerol

A208C 2 × 1ml

SDS

Choose language:

Download SDSPDF (279 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

CC

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