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CytoTox-Glo™ Cytotoxicity Assay

Measures Cytotoxicity Due to Loss of Membrane Integrity

  • Measures extracellular activity of dead-cell protease
  • Easy and fast "add-mix-measure" protocol
  • Powerful luminescent detection

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Catalog number selected: G9290

$ 131.00
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CytoTox-Glo™ Cytotoxicity Assay
10ml
$ 131.00
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Measure Relative Dead Cell Number in Cell Populations 

The CytoTox-Glo™ Assay is a luminescent cytotoxicity assay that measures the relative number of dead cells in cell populations. The assay measures the extracellular activity of a distinct intracellular protease activity (dead-cell protease) when the protease is released from membrane-compromised cells. A luminogenic cell-impermeant peptide substrate (AAF-aminoluciferin) is used to measure dead-cell protease activity. The liberated aminoluciferin product is measured as "glow type" luminescence generated by Ultra-Glo™ Recombinant Luciferase provided in the assay reagent. The AAF-aminoluciferin substrate cannot cross the intact membrane of viable cells and does not generate any appreciable signal from the live-cell population. The amount of luminescence directly correlates with the percentage of cells undergoing cytotoxic stress.

With the addition of a lysis reagent (provided), the CytoTox-Glo™ Assay also can deliver the luminescent signal associated with the total number of cells in each assay well. Viability can be calculated by subtracting the luminescent dead-cell signal from the total luminescent value, thus allowing you to normalize assay data to cell number and mitigate assay interferences that may lead to erroneous conclusions. The cytotoxicity protease biomarker is constitutive and conserved across cell lines, and the CytoTox-Glo™ Assay demonstrates excellent correlation with other methods of assessing cell viability.

6393mb-vs2

Highly Sensitive

6973MA-mod

The CytoTox-Glo™ Assay detects small changes in viability because of its high sensitivity. This graph shows the superior signal-to-noise ratios of the CytoTox-Glo™ Assay compared to a fluorescent LDH assay. Detects as few as 200-500 cytotoxic cells in a total population of 10,000 cells and less than 10 cytotoxic cells in a limiting dilution series.

More Reliable Data

6552MA-mod

Determining cytotoxicity and viability by lysis. The luminescent signal collected after lysis was adjusted to reflect the "live cell" contribution by subtracting the initial dead cell signal. The data above are plotted as "dead cell" or "live cell" signal versus the concentration of calcium ionophore.

Notes

Using 100μl/assay in a 96-well plate format, Cat.# G9290 is sufficient to perform 100 assays; Cat.# G9291, 500 assays; Cat.# G9292, 1,000 assays. Using 25μl per well in a 384-well plate format, Cat.# G9290 is sufficient to perform 400 assays; Cat.# G9291, 2,000 assays; Cat.# G9292, 4,000 assays.

Specifications

You are viewing: G9290 Change Configuration

What's in the box?

Item Part # Size

Assay Buffer

G146A 2 × 5ml

AAF-Glo™ Substrate

G607A 1 × 1 bottle

Digitonin

G944A 1 × 40μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Patent Pending.

Specifications

You are viewing: G9291 Change Configuration

What's in the box?

Item Part # Size

Assay Buffer

G146A 10 × 5ml

AAF-Glo™ Substrate

G607A 5 × 1 bottle

Digitonin

G944B 1 × 175μl

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

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Search

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Patent Pending.

Specifications

You are viewing: G9292 Change Configuration

What's in the box?

Item Part # Size

Assay Buffer

G146B 4 × 25ml

AAF-Glo™ Substrate

G607B 2 × 1 bottle

Digitonin

G944B 2 × 175μl

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for G9292

Search

View more results

No results

Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Patent Pending.

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