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Applications of a Smaller Brighter More Versatile Luciferase Reporter

Kyle received his Ph.D. in Biochemistry from the University of Kansas Medical Center where he studied the interactions of prolactin with its receptor using techniques like cell-based assays, protein expression, and molecular biology. Prior to joining Promega, he did a post-doc at the University of Texas Southwestern Medical Center in the Molecular Department. Kyle studied the interaction of diphtheria toxin with its receptor, heparin-binding EGF-like growth factor, using techniques like site-directed mutagenesis, transfection and cell-based assays. Kyle first worked for five and a half years in the Promega Technical Services department helping researchers troubleshoot and refine their assays. Next, Kyle moved into product development working closely with R&D and recently joined the newly formed North American branch office focusing on cellular analysis and personal automation.

  • Kyle Hooper, Ph.D.

  • Original Webinar Date: Tuesday, November 13, 2012

Abstract

NanoLuc™ Luciferase, a 19kDa, monomeric luciferase gives a brighter signal and enables more sensitive measurement. The NanoLuc™/Nano-Glo™ Assay is ~100-fold brighter than either firefly or Renilla luciferase in vitro and in cells.

This seminar will address:

  • Characteristics and uses of NanoLuc™ Luciferase
  • Strategies for handling the bright luminescence
  • Considerations for choosing Firefly or NanoLuc™ Luciferase
  • Applications of NanoLuc™ Luciferase as a fusion partner for:
    • Protein translocation/degradation
    • Protein/Protein interactions (utilizing BRET)
    • Intracellular biosensors (utilizing BRET)

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