The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity.
Bricambert, J., Alves-Guerra, M.C., Esteves, P., Prip-Buus, C., Bertrand-Michel, J., Guillou, H., Chang, C.J., Vander Wal, M.N., Canonne-Hergaux, F., Mathurin, P., Raverdy, V., Pattou, F., Girard, J., Postic, C. and Dentin, R.
Notes: The Nrf2-response element luciferase was used to measure transcriptional activity of a novel transcriptional activator Plant Homeodomain Finger 2 (Phf2). Interestingly, Phf2 was identified to protect the liver from Non Alcoholic Fatty Liver Disease (NAFLD) through histone demethylation. CGSH/GSSG-Glo™ was used to monitor glutathione content in the liver, while the ApoTox-Glo™ assay was used to monitor for cell viability and toxicity. (5179)
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