Costa, R.C., Mesquita, L.P., Nunes, M.V.L., Oliveira, I.M., Oliveira, L.F.S., Souza, A.R., Maiorka, P.C. and Varaschin, M.S.
Notes: Reliaprep gDNA Tissue Miniprep System was used to extract DNA from the placentas and gerbil tissues (brain, pancreas, liver, spleen, heart and skeletal muscle). Then PCR was performed to detect the presence or absence of N. caninum NC5 gene and serum samples were collected for the indirect fluorescent antibody test using non-Promega Products. (4978)