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Rat Hepatocyte Culture Physiology Shows Enhanced Cytochrome P450 Activity on a Synthetic Extracellular Matrix

Mark E. Rothenberg1, James J. Cali2, Mary Sobol2, Michael W. Briggs1 and Todd Upton1
1Technical Applications, Corning Life Sciences, 2Promega Corporation
Publication Date: 2008

Abstract

We examined the impact of a new three-dimensional synthetic matrix on hepatocyte physiology. Hepatocytes were plated on Corning labware with Ultra-Web™ and Ultra-Web™ polyamine surfaces and on Becton Dickinson (BD) BioCoat™ collagen I microplates. The samples then were challenged for 48 hours with two known modulators of cytochrome P450, and P450 activity was measured using the P450-Glo® Assay. The data show enhanced physiologic function of primary rat hepatocytes on microplates coated with the Ultra-Web™ synthetic matrix.

Promega Notes 99, 29–31.

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